Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Physiol Rep
2022 Jan 01;101:e15164. doi: 10.14814/phy2.15164.
Show Gene links
Show Anatomy links
Boric acid transport activity of human aquaporins expressed in Xenopus oocytes.
Ushio K
,
Watanabe E
,
Kamiya T
,
Nagashima A
,
Furuta T
,
Imaizumi G
,
Fujiwara T
,
Romero MF
,
Kato A
.
???displayArticle.abstract???
Boric acid is a vital micronutrient that is toxic at high concentrations in animals. However, the mechanisms underlying boric acid transport in animal cells remain unclear. To identify the plasma membrane boric acid channels in animals, we analyzed the function of human aquaporins (AQPs), which are homologous to the nodulin-like intrinsic protein family of plant boric acid channels. When human AQPs were expressed in Xenopus laevis oocytes, the results of the swelling assay showed that boric acid permeability significantly increased in oocytes expressing AQP3, 7, 8, 9, and 10, but not in those expressing AQP1, 2, 4, and 5. The boric acid influxes of these oocytes were also confirmed by elemental quantification. Electrophysiological analysis using a pH microelectrode showed that these AQPs transported boric acid (B(OH)3 ) but not borate ions (B(OH)4- ). These results indicate that AQP3, 7, 8, 9, and 10 act as boric acid transport systems, likely as channels in humans.
Agre,
Aquaporin water channels--from atomic structure to clinical medicine.
2002, Pubmed
Agre,
Aquaporin water channels--from atomic structure to clinical medicine.
2002,
Pubmed
Ampah-Korsah,
The Aquaporin Splice Variant NbXIP1;1α Is Permeable to Boric Acid and Is Phosphorylated in the N-terminal Domain.
2016,
Pubmed
Azad,
Human Aquaporins: Functional Diversity and Potential Roles in Infectious and Non-infectious Diseases.
2021,
Pubmed
Beitz,
Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia, and protons.
2006,
Pubmed
,
Xenbase
Blevins,
BORON IN PLANT STRUCTURE AND FUNCTION.
1998,
Pubmed
Borgnia,
Cellular and molecular biology of the aquaporin water channels.
1999,
Pubmed
de Maré,
Structural Basis for Glycerol Efflux and Selectivity of Human Aquaporin 7.
2020,
Pubmed
Frick,
X-ray structure of human aquaporin 2 and its implications for nephrogenic diabetes insipidus and trafficking.
2014,
Pubmed
Gotfryd,
Human adipose glycerol flux is regulated by a pH gate in AQP10.
2018,
Pubmed
Hibuse,
Aquaporins and glycerol metabolism.
2006,
Pubmed
Ishibashi,
Cloning and functional expression of a new aquaporin (AQP9) abundantly expressed in the peripheral leukocytes permeable to water and urea, but not to glycerol.
1998,
Pubmed
,
Xenbase
Ishibashi,
Cloning and identification of a new member of water channel (AQP10) as an aquaglyceroporin.
2002,
Pubmed
,
Xenbase
Jumper,
Highly accurate protein structure prediction with AlphaFold.
2021,
Pubmed
Kao,
SLC4A11 function: evidence for H+(OH-) and NH3-H+ transport.
2020,
Pubmed
Koyama,
Cloning and functional expression of human aquaporin8 cDNA and analysis of its gene.
1998,
Pubmed
,
Xenbase
Kuwahara,
Mercury-sensitive residues and pore site in AQP3 water channel.
1997,
Pubmed
,
Xenbase
Laforenza,
Mammalian aquaglyceroporin function in metabolism.
2016,
Pubmed
Lee,
The human Aquaporin-5 gene. Molecular characterization and chromosomal localization.
1996,
Pubmed
,
Xenbase
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Litman,
Ammonia and urea permeability of mammalian aquaporins.
2009,
Pubmed
Liu,
Purification and functional characterization of aquaporin-8.
2006,
Pubmed
Loganathan,
Functional assessment of SLC4A11, an integral membrane protein mutated in corneal dystrophies.
2016,
Pubmed
,
Xenbase
Lopalco,
Boric Acid, a Lewis Acid With Unique and Unusual Properties: Formulation Implications.
2020,
Pubmed
Marsiccobetre,
Aquaglyceroporins Are the Entry Pathway of Boric Acid in Trypanosoma brucei.
2017,
Pubmed
,
Xenbase
Misaka,
A water channel closely related to rat brain aquaporin 4 is expressed in acid- and pepsinogen-secretory cells of human stomach.
1996,
Pubmed
,
Xenbase
Miwa,
Boron transport in plants: co-ordinated regulation of transporters.
2010,
Pubmed
Mobasheri,
Distribution of AQP2 and AQP3 water channels in human tissue microarrays.
2005,
Pubmed
Moss,
Aquaporin-7: A Dynamic Aquaglyceroporin With Greater Water and Glycerol Permeability Than Its Bacterial Homolog GlpF.
2020,
Pubmed
,
Xenbase
Murray,
A comparative review of the pharmacokinetics of boric acid in rodents and humans.
1998,
Pubmed
Myers,
Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH.
2016,
Pubmed
,
Xenbase
Nielsen,
Update on human health effects of boron.
2014,
Pubmed
Nielsen,
Aquaporins in the kidney: from molecules to medicine.
2002,
Pubmed
Nozawa,
Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae.
2006,
Pubmed
Ogando,
SLC4A11 is an EIPA-sensitive Na(+) permeable pHi regulator.
2013,
Pubmed
Park,
NaBC1 is a ubiquitous electrogenic Na+ -coupled borate transporter essential for cellular boron homeostasis and cell growth and proliferation.
2004,
Pubmed
,
Xenbase
Parker,
Human BTR1, a new bicarbonate transporter superfamily member and human AE4 from kidney.
2001,
Pubmed
Pravda,
MOLEonline: a web-based tool for analyzing channels, tunnels and pores (2018 update).
2018,
Pubmed
Preston,
Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein.
1992,
Pubmed
,
Xenbase
Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase
Romero,
Expression cloning using Xenopus laevis oocytes.
1998,
Pubmed
,
Xenbase
Sasaki,
Cloning, characterization, and chromosomal mapping of human aquaporin of collecting duct.
1994,
Pubmed
,
Xenbase
Sciortino,
Cation and voltage dependence of rat kidney electrogenic Na(+)-HCO(-)(3) cotransporter, rkNBC, expressed in oocytes.
1999,
Pubmed
,
Xenbase
Sievers,
Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega.
2011,
Pubmed
Takano,
Arabidopsis boron transporter for xylem loading.
2002,
Pubmed
Tanaka,
Physiological roles and transport mechanisms of boron: perspectives from plants.
2008,
Pubmed
Tingaud-Sequeira,
The zebrafish genome encodes the largest vertebrate repertoire of functional aquaporins with dual paralogy and substrate specificities similar to mammals.
2010,
Pubmed
,
Xenbase
Tsukaguchi,
Molecular characterization of a broad selectivity neutral solute channel.
1998,
Pubmed
,
Xenbase
Uluisik,
The importance of boron in biological systems.
2018,
Pubmed
Wang,
What makes an aquaporin a glycerol channel? A comparative study of AqpZ and GlpF.
2005,
Pubmed
Zhu,
Expression, Distribution and Role of Aquaporin Water Channels in Human and Animal Stomach and Intestines.
2016,
Pubmed
