Pest Manag Sci 2022 Nov 01;7811:4963-4974. doi: 10.1002/ps.7119.

Atrazine and estradiol effects on development of Acris blanchardi (Blanchard's cricket frog) exposed in outdoor enclosures.

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BACKGROUND: The herbicide atrazine has been proposed as a potential endocrine disrupting compound (EDC) for amphibians. Using atrazine concentrations below or at those typically found in surface waters (0.5, 5.0, 50 μg/L), we exposed Acris blanchardi (Blanchard's cricket frog) larvae throughout development until metamorphosis (i.e. Gosner stages 26-45). An additional 50 μg/L treatment (50s μg/L) was utilized where supplemented algae was added to control for indirect atrazine effects from reduced food sources. In addition to atrazine, experimental groups also included a negative control and two positive controls, 17β-estradiol (E2) at 2.3 and 25 μg/L. At 60 days post-metamorphosis, A. blanchardi metamorphs were euthanized for analysis of gross and histopathological development. RESULTS: Atrazine did not significantly influence mortality (mean recovery of 54% across treatments), sex ratio, body mass (BM), snout-vent length (SVL), gonad size, nor gonad development of A. blanchardi. Females exposed to 50s μg/L atrazine had 29% less mass, were 10% shorter, and had a 29% lower mean ovary area (mm2 ) as compared to negative controls, suggesting algae enrichment had a significant negative effect. Males exposed to estradiol (25 μg/L) showed an increased level of oviduct development. Ovary area was also significantly influenced by estradiol treatment at 2.3 and 25 μg/L. CONCLUSION: Overall, estradiol had much less effect than predicted based on other model species (e.g. Xenopus laevis). Development of A. blanchardi, overall, was not affected by long-term exposure to environmentally relevant concentrations of atrazine. However, this species also was largely insensitive to exogenous estradiol in this test system. © 2022 Society of Chemical Industry.

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Species referenced: Xenopus laevis