Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Sci Rep
2022 Dec 03;121:20934. doi: 10.1038/s41598-022-25105-5.
Show Gene links
Show Anatomy links
Sex-linked markers in an Australian frog Platyplectrum ornatum (Limnodynastidae) with a small genome and homomorphic sex chromosomes.
Schimek C
,
Shams F
,
Miura I
,
Clulow S
,
Majtanova Z
,
Deakin J
,
Ezaz T
.
???displayArticle.abstract???
Amphibians have highly diverse sex-determining modes leading to a notable interest in vertebrate sex determination and sex chromosome evolution. The identification of sex-determining systems in amphibians, however, is often difficult as a vast majority consist of homomorphic sex chromosomes making them hard to distinguish. In this study, we used Diversity Array Technology sequencing (DArTseq) to identify the sex-determining system in the ornate burrowing frog from Australia, Platyplectrum ornatum. We applied DArTseq to 44 individuals, 19 males and 25 females, collected from two locations to develop sex-linked markers. Unexpectedly, these 44 individuals were classified into two distinct population clusters based on our SNP analyses, 36 individuals in cluster 1, and 8 individuals in cluster 2. We then performed sex-linkage analyses separately in each cluster. We identified 35 sex-linked markers from cluster 1, which were all associated with maleness. Therefore, P. ornatum cluster 1 is utilising a male heterogametic (XX/XY) sex-determining system. On the other hand, we identified 210 sex-linked markers from cluster 2, of which 89 were male specific, i.e., identifying XX/XY sex determining system and 111 were female specific, i.e., identifying ZZ/ZW sex determining system, suggesting existence of either male or female heterogametic sex determining system in cluster 2. We also performed cytogenetic analyses in 1 male and 1 female from cluster 1; however, we did not detect any visible differentiation between the X and Y sex chromosomes. We also mapped sex-linked markers from the two clusters against the P. ornatum genome and our comparative analysis indicated that the sex chromosomes in both clusters shared homologies to chromosome 10 (autosome) of Rana temporaria and ZWY sex chromosome of Xenopus tropicalis. Our preliminary data suggest that it is plausible that the cluster 2 has a potential to be either male or female heterogamety in sex determination, requiring further investigation.
Bachtrog,
Sex determination: why so many ways of doing it?
2014, Pubmed
Bachtrog,
Sex determination: why so many ways of doing it?
2014,
Pubmed
Capel,
Vertebrate sex determination: evolutionary plasticity of a fundamental switch.
2017,
Pubmed
de Cáscia Barreto Netto,
A standard protocol for obtaining fish chromosomes under post-mortem conditions.
2007,
Pubmed
Ezaz,
Relationships between vertebrate ZW and XY sex chromosome systems.
2006,
Pubmed
Ezaz,
The dragon lizard Pogona vitticeps has ZZ/ZW micro-sex chromosomes.
2005,
Pubmed
Furman,
A frog with three sex chromosomes that co-mingle together in nature: Xenopus tropicalis has a degenerate W and a Y that evolved from a Z chromosome.
2020,
Pubmed
,
Xenbase
Gruber,
dartr: An r package to facilitate analysis of SNP data generated from reduced representation genome sequencing.
2018,
Pubmed
Hill,
Conservation of Sex-Linked Markers among Conspecific Populations of a Viviparous Skink, Niveoscincus ocellatus, Exhibiting Genetic and Temperature-Dependent Sex Determination.
2018,
Pubmed
Jeffries,
A rapid rate of sex-chromosome turnover and non-random transitions in true frogs.
2018,
Pubmed
Kearse,
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.
2012,
Pubmed
Kumar,
TimeTree: A Resource for Timelines, Timetrees, and Divergence Times.
2017,
Pubmed
Lambert,
Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution.
2016,
Pubmed
Lamichhaney,
A bird-like genome from a frog: Mechanisms of genome size reduction in the ornate burrowing frog, Platyplectrum ornatum.
2021,
Pubmed
Ma,
The Diversity and Evolution of Sex Chromosomes in Frogs.
2021,
Pubmed
Miura,
The late replication banding patterns of chromosomes are highly conserved in the genera Rana, Hyla, and Bufo (Amphibia: Anura).
1995,
Pubmed
Miura,
An evolutionary witness: the frog rana rugosa underwent change of heterogametic sex from XY male to ZW female.
2007,
Pubmed
Miura,
Evolution of a Multiple Sex-Chromosome System by Three-Sequential Translocations among Potential Sex-Chromosomes in the Taiwanese Frog Odorrana swinhoana.
2021,
Pubmed
Miura,
Sex Determination and Sex Chromosomes in Amphibia.
2017,
Pubmed
Ogata,
The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa.
2008,
Pubmed
Roco,
Coexistence of Y, W, and Z sex chromosomes in Xenopus tropicalis.
2015,
Pubmed
,
Xenbase
Rodrigues,
Geographic variation in sex-chromosome differentiation in the common frog (Rana temporaria).
2014,
Pubmed
Ruiz-García,
Sex Differentiation in Amphibians: Effect of Temperature and Its Influence on Sex Reversal.
2021,
Pubmed
Saidapur,
Effect of sex steroids on gonadal differentiation and sex reversal in the frog, Rana curtipes.
2001,
Pubmed
Sakurai,
Immunohistochemical detection and biological activities of CYP17 (P450c17) in the indifferent gonad of the frog Rana rugosa.
2008,
Pubmed
Sarre,
Transitions between sex-determining systems in reptiles and amphibians.
2011,
Pubmed
Shams,
Application of DArT seq derived SNP tags for comparative genome analysis in fishes; An alternative pipeline using sequence data from a non-traditional model species, Macquaria ambigua.
2019,
Pubmed
Sopniewski,
Identifying sex-linked markers in Litoria aurea: a novel approach to understanding sex chromosome evolution in an amphibian.
2019,
Pubmed
Suda,
Molecular cytogenetic characterization of telomere-specific repetitive DNA sequences in Rana rugosa.
2011,
Pubmed
Toups,
A reciprocal translocation radically reshapes sex-linked inheritance in the common frog.
2019,
Pubmed
Uno,
Extraordinary Diversity in the Origins of Sex Chromosomes in Anurans Inferred from Comparative Gene Mapping.
2015,
Pubmed
,
Xenbase
Xu,
Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers.
2022,
Pubmed
Yoshimoto,
A W-linked DM-domain gene, DM-W, participates in primary ovary development in Xenopus laevis.
2008,
Pubmed
,
Xenbase
Yoshimoto,
Opposite roles of DMRT1 and its W-linked paralogue, DM-W, in sexual dimorphism of Xenopus laevis: implications of a ZZ/ZW-type sex-determining system.
2010,
Pubmed
,
Xenbase
