Locascio A , Annona G , Caccavale F , D'Aniello S , Agnisola C , Palumbo A .

             nitric oxide homeostasis

	Figure 1. Schematic resuming of all major transformations putatively involved in nitric oxide (NO) homeostasis. Under normoxic conditions, NO is synthesized by Nos enzymes from L-arginine. In organisms possessing the ornithine–urea cycle (OUC), the co-product of the reaction, L-citrulline, is reconverted to L-arginine (the citrulline–NO cycle). NO signaling may occur by (i) forming an iron-nitrosyl complex with hemoproteins; (ii) promoting protein post-translational modifications via S-nitrosylation, forming S-nitroso compounds with thiol residues (SNO); and (iii) forming N-nitroso (NNO) compounds. NO can be (i) rapidly converted to nitrite with dissolved O2 by NO scavenging reactions or can be (ii) inactivated to nitrate by oxygenated heme proteins. Excess NO (iii) can react with superoxide to form peroxynitrite (ONOO−), which in turn can interact with lipids, DNA, and proteins to give nitrated derivatives or (iv) react with oxygen to form N2O3, which interacts with thiol groups to give nitrosylated proteins. When de novo production via Nos is compromised under low O2, NO can be produced by nitrite reduction in heme proteins (the globin–NO cycle), possibly associated with nitrate conversion to nitrite by xanthine oxidoreductase. The interactions with arginine and protein homeostasis may be significant in determining the balance between NO formation and scavenging.
	Figure 2. Nos repertoire in deuterostomes. Silhouettes are from PhyloPic (PhyloPic—free silhouette images of life forms) and represent the following: (1) Human (Homo sapiens); (2) Frog (Xenopus laevis and Xenopus tropicalis); (3) Chicken (Gallus gallus); (4) Lizard (Anolis carolinensis); (5) Coelacanth (Latimeria chalumnae); (6) Medaka (Oryzias latipes); (7) Carp (Carassius auratus, Cyprinus carpio, and Sinocyclocheilus anshuiensis); (8) Zebrafish (Danio rerio); (9) Northern pike (Esox lucius); (10) Salmon (Oncorhynchus mykiss and Salmo salar); (11) Elephant fish (Paramormyrops kingsleyae); (12) Spotted gar (Lepisosteus oculatus); (13) Bowfin (Amia calva); (14) Sturgeon (Acipenser ruthenus); (15) Bichir (Polypterus senegalus); (16) Shark (Scyliorhinus torazame); (17) Lamprey (Lethenteron camtschaticum, Petromyzon marinus); (18) Sea squirt (Ciona robusta and Ciona savignyi); (19) Amphioxus (Asymmetron lucayanum, Branchiostoma belcheri, Branchiostoma floridae, and Branchiostoma lanceolatum); (20) Sea urchin (Hemicentrotus pulcherrimus and Strongylocentrotus purpuratus). Abbreviations: Cs4R, carp-specific genome duplication; Ss4R, salmonid-specific genome duplication; TGD, teleost genome duplication; VGD, vertebrate genome duplication.
	Figure 3. Comparison of the major physiological functions of NO in aquatic chordates. The NO roles in immunity, reproduction, feeding and gut function, neuro-modulation, and development are common to all chordates (green). The role in locomotion is conserved among vertebrates and tunicates (lilac), but no data are available in cephalochordates (question mark). NO function in metamorphosis is common to all chordates with an indirect development (light brown). Cardio-respiratory modulation by NO is reported in vertebrates and tunicates (orange).

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