XB-ART-60574
Cell Rep
2019 Dec 24;2913:4583-4592.e3. doi: 10.1016/j.celrep.2019.11.107.
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Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.
Rathore SS
,
Liu Y
,
Yu H
,
Wan C
,
Lee M
,
Yin Q
,
Stowell MHB
,
Shen J
.
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Intracellular vesicle fusion is mediated by soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18 (SM) proteins. It is generally accepted that membrane fusion occurs when the vesicle and target membranes are brought into close proximity by SNAREs and SM proteins. In this work, we demonstrate that, for fusion to occur, membrane bilayers must be destabilized by a conserved membrane-embedded motif located at the juxtamembrane region of the vesicle-anchored v-SNARE. Comprised of basic and hydrophobic residues, the juxtamembrane motif perturbs the lipid bilayer structure and promotes SNARE-SM-mediated membrane fusion. The juxtamembrane motif can be functionally substituted with an unrelated membrane-disrupting peptide in the membrane fusion reaction. These findings establish the juxtamembrane motif of the v-SNARE as a membrane-destabilizing peptide. Requirement of membrane-destabilizing peptides is likely a common feature of biological membrane fusion.
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???displayArticle.grants??? [+]
R01 DK095367 NIDDK NIH HHS , R35 GM126960 NIGMS NIH HHS , R01 DK124431 NIDDK NIH HHS , R56 DK095367 NIDDK NIH HHS , R03 AI135473 NIAID NIH HHS , RF1 AG061829 NIA NIH HHS
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