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The Bmp/Smad1 pathway plays a crucial role in developmental processes and tissue homeostasis. Mitogen-activated protein kinase (Mapk)/Erk mediated phosphorylation of Smad1 in the linker region leads to Smad1 degradation, cytoplasmic retention and inhibition of Bmp/Smad1 signaling. While Fgf/Erk pathway has been documented to inhibit Bmp/Smad1 signaling, several studies also suggests the cooperative interaction between these two pathways in different context. However, the precise role and molecular pathway of this collaborative interaction remain obscure. Here, we identified Xbra induced by Fgf/Erk signaling as a factor in a protective mechanism for Smad1. Xbra physically interacted with the linker region phosphorylated Smad1 to make Xbra/Smad1/Smad4 trimeric complex, leading to Smad1 nuclear localization and protecting it from ubiquitin-mediated proteasomal degradation. This interaction of Xbra/Smad1/Smad4 led to sustained nuclear localization of Smad1 and the upregulation of lateralmesoderm genes, while concurrently suppression of neural and blood forming genes. Taken together, the results suggests Xbra-dependent cooperative interplays between Fgf/Erk and Bmp/Smad1 signaling during lateralmesoderm specification in Xenopus embryos.
2018M3C7A1056285 National Research Foundation of Korea, 2021R1A4A1027355 National Research Foundation of Korea, 2021M3H9A1097557 National Research Foundation of Korea
Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993, Pubmed,
Xenbase
Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993,
Pubmed
,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Beck,
The role of BMP signaling in outgrowth and patterning of the Xenopus tail bud.
2001,
Pubmed
,
Xenbase
Chen,
Bone morphogenetic proteins.
2004,
Pubmed
Chen,
Finale: the last minutes of Smads.
2009,
Pubmed
Dale,
BMP signalling in early Xenopus development.
1999,
Pubmed
,
Xenbase
De Robertis,
Dorsal-ventral patterning and neural induction in Xenopus embryos.
2004,
Pubmed
,
Xenbase
Demagny,
The tumor suppressor Smad4/DPC4 is regulated by phosphorylations that integrate FGF, Wnt, and TGF-β signaling.
2014,
Pubmed
,
Xenbase
Dosch,
Bmp-4 acts as a morphogen in dorsoventral mesoderm patterning in Xenopus.
1997,
Pubmed
,
Xenbase
Fisher,
eFGF is required for activation of XmyoD expression in the myogenic cell lineage of Xenopus laevis.
2002,
Pubmed
,
Xenbase
Fletcher,
FGF8 spliceforms mediate early mesoderm and posterior neural tissue formation in Xenopus.
2006,
Pubmed
,
Xenbase
Godin,
Role of BMP family members during kidney development.
1999,
Pubmed
Harland,
Formation and function of Spemann's organizer.
1997,
Pubmed
Harvey,
MyoD protein expression in Xenopus embryos closely follows a mesoderm induction-dependent amplification of MyoD transcription and is synchronous across the future somite axis.
1992,
Pubmed
,
Xenbase
Heasman,
Patterning the Xenopus blastula.
1997,
Pubmed
,
Xenbase
Hoppler,
BMP-2/-4 and Wnt-8 cooperatively pattern the Xenopus mesoderm.
1998,
Pubmed
,
Xenbase
Hopwood,
MyoD expression in the forming somites is an early response to mesoderm induction in Xenopus embryos.
1989,
Pubmed
,
Xenbase
Isaacs,
Expression of a novel FGF in the Xenopus embryo. A new candidate inducing factor for mesoderm formation and anteroposterior specification.
1992,
Pubmed
,
Xenbase
Isaacs,
eFGF regulates Xbra expression during Xenopus gastrulation.
1994,
Pubmed
,
Xenbase
Isaacs,
FGF4 regulates blood and muscle specification in Xenopus laevis.
2007,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Kumano,
Revisions to the Xenopus gastrula fate map: implications for mesoderm induction and patterning.
2002,
Pubmed
,
Xenbase
Kumano,
Spatial and temporal properties of ventral blood island induction in Xenopus laevis.
1999,
Pubmed
,
Xenbase
Kumano,
FGF signaling restricts the primary blood islands to ventral mesoderm.
2000,
Pubmed
,
Xenbase
Kumar,
Xbra and Smad-1 cooperate to activate the transcription of neural repressor ventx1.1 in Xenopus embryos.
2018,
Pubmed
,
Xenbase
Lane,
The origins of primitive blood in Xenopus: implications for axial patterning.
1999,
Pubmed
,
Xenbase
Lee,
Direct response elements of BMP within the PV.1A promoter are essential for its transcriptional regulation during early Xenopus development.
2011,
Pubmed
,
Xenbase
Makanae,
Co-operative Bmp- and Fgf-signaling inputs convert skin wound healing to limb formation in urodele amphibians.
2014,
Pubmed
Mills,
Elucidating the origins of the vascular system: a fate map of the vascular endothelial and red blood cell lineages in Xenopus laevis.
1999,
Pubmed
,
Xenbase
Pera,
Integration of IGF, FGF, and anti-BMP signals via Smad1 phosphorylation in neural induction.
2003,
Pubmed
,
Xenbase
Pyati,
Transgenic zebrafish reveal stage-specific roles for Bmp signaling in ventral and posterior mesoderm development.
2005,
Pubmed
Sapkota,
Balancing BMP signaling through integrated inputs into the Smad1 linker.
2007,
Pubmed
,
Xenbase
Schulte-Merker,
Mesoderm formation in response to Brachyury requires FGF signalling.
1995,
Pubmed
,
Xenbase
Sharma,
Bmp signaling maintains a mesoderm progenitor cell state in the mouse tailbud.
2017,
Pubmed
Shi,
Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
2003,
Pubmed
Standley,
eFGF and its mode of action in the community effect during Xenopus myogenesis.
2001,
Pubmed
,
Xenbase
Stickney,
Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates.
2007,
Pubmed
Tracey,
A Xenopus homologue of aml-1 reveals unexpected patterning mechanisms leading to the formation of embryonic blood.
1998,
Pubmed
,
Xenbase
Verheyen,
Opposing effects of Wnt and MAPK on BMP/Smad signal duration.
2007,
Pubmed
Winnier,
Bone morphogenetic protein-4 is required for mesoderm formation and patterning in the mouse.
1995,
Pubmed
Xu,
Opposite effects of FGF and BMP-4 on embryonic blood formation: roles of PV.1 and GATA-2.
1999,
Pubmed
,
Xenbase
Yoon,
PV.1 induced by FGF-Xbra functions as a repressor of neurogenesis in Xenopus embryos.
2014,
Pubmed
,
Xenbase
