Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A vertebrate Vangl2 translational variant required for planar cell polarity.
Walton A
,
Thomé V
,
Revinski D
,
Marchetto S
,
Puvirajesinghe TM
,
Audebert S
,
Camoin L
,
Bailly E
,
Kodjabachian L
,
Borg JP
.
???displayArticle.abstract???
First described in the milkweed bug Oncopeltus fasciatus, planar cell polarity (PCP) is a developmental process essential for embryogenesis and development of polarized structures in Metazoans. This signaling pathway involves a set of evolutionarily conserved genes encoding transmembrane (Vangl, Frizzled, Celsr) and cytoplasmic (Prickle, Dishevelled) molecules. Vangl2 is of major importance in embryonic development as illustrated by its pivotal role during neural tube closure in human, mouse, Xenopus, and zebrafish embryos. Here, we report on the molecular and functional characterization of a Vangl2 isoform, Vangl2-Long, containing an N-terminal extension of about 50 aa, which arises from an alternative near-cognate AUA translation initiation site, lying upstream of the conventional start codon. While missing in Vangl1 paralogs and in all invertebrates, including Drosophila, this N-terminal extension is conserved in all vertebrate Vangl2 sequences. We show that Vangl2-Long belongs to a multimeric complex with Vangl1 and Vangl2. Using morpholino oligonucleotides to specifically knockdown Vangl2-Long in Xenopus, we found that this isoform is functional and required for embryo extension and neural tube closure. Furthermore, both Vangl2 and Vangl2-Long must be correctly expressed for the polarized distribution of the PCP molecules Pk2 and Dvl1 and for centriole rotational polarity in ciliated epidermal cells. Altogether, our study suggests that Vangl2-Long significantly contributes to the pool of Vangl2 molecules present at the plasma membrane to maintain PCP in vertebrate tissues.
Acland,
Subcellular fate of the int-2 oncoprotein is determined by choice of initiation codon.
1990, Pubmed
Acland,
Subcellular fate of the int-2 oncoprotein is determined by choice of initiation codon.
1990,
Pubmed
Adilakshmi,
A nuclear variant of ErbB3 receptor tyrosine kinase regulates ezrin distribution and Schwann cell myelination.
2011,
Pubmed
Basta,
New mouse models for high resolution and live imaging of planar cell polarity proteins in vivo.
2021,
Pubmed
Belotti,
Molecular characterisation of endogenous Vangl2/Vangl1 heteromeric protein complexes.
2012,
Pubmed
Bueno,
Evolutionary development of embryonic cerebrospinal fluid composition and regulation: an open research field with implications for brain development and function.
2016,
Pubmed
Butler,
Control of vertebrate core planar cell polarity protein localization and dynamics by Prickle 2.
2015,
Pubmed
,
Xenbase
Chu,
Wnt proteins can direct planar cell polarity in vertebrate ectoderm.
2016,
Pubmed
,
Xenbase
Ciruna,
Planar cell polarity signalling couples cell division and morphogenesis during neurulation.
2006,
Pubmed
Dai,
Identification and characterization of a new isoform of small GTPase RhoE.
2020,
Pubmed
Darken,
The planar polarity gene strabismus regulates convergent extension movements in Xenopus.
2002,
Pubmed
,
Xenbase
Gao,
Wnt signaling gradients establish planar cell polarity by inducing Vangl2 phosphorylation through Ror2.
2011,
Pubmed
Goto,
The planar cell polarity gene strabismus regulates convergence and extension and neural fold closure in Xenopus.
2002,
Pubmed
,
Xenbase
Gravel,
Molecular and cellular mechanisms underlying neural tube defects in the loop-tail mutant mouse.
2010,
Pubmed
Hopkins,
A secreted PTEN phosphatase that enters cells to alter signaling and survival.
2013,
Pubmed
Huez,
New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation.
2001,
Pubmed
Iliescu,
Transmembrane topology of mammalian planar cell polarity protein Vangl1.
2011,
Pubmed
Ivanov,
Identification of evolutionarily conserved non-AUG-initiated N-terminal extensions in human coding sequences.
2011,
Pubmed
Jessen,
Identification and developmental expression pattern of van gogh-like 1, a second zebrafish strabismus homologue.
2004,
Pubmed
Jessen,
Zebrafish trilobite identifies new roles for Strabismus in gastrulation and neuronal movements.
2002,
Pubmed
Jussila,
Live imaging and conditional disruption of native PCP activity using endogenously tagged zebrafish sfGFP-Vangl2.
2022,
Pubmed
Kibar,
Mutations in VANGL1 associated with neural-tube defects.
2007,
Pubmed
Kibar,
Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail.
2001,
Pubmed
Kobayashi,
Targeted mass spectrometric analysis of N-terminally truncated isoforms generated via alternative translation initiation.
2009,
Pubmed
Lei,
VANGL2 mutations in human cranial neural-tube defects.
2010,
Pubmed
Liang,
PTENα, a PTEN isoform translated through alternative initiation, regulates mitochondrial function and energy metabolism.
2014,
Pubmed
López-Iniesta,
Conserved Double Translation Initiation Site for Δ160p53 Protein Hints at Isoform's Key Role in Mammalian Physiology.
2022,
Pubmed
Marchal,
BMP inhibition initiates neural induction via FGF signaling and Zic genes.
2009,
Pubmed
,
Xenbase
Mitchell,
The PCP pathway instructs the planar orientation of ciliated cells in the Xenopus larval skin.
2009,
Pubmed
,
Xenbase
Murdoch,
Severe neural tube defects in the loop-tail mouse result from mutation of Lpp1, a novel gene involved in floor plate specification.
2001,
Pubmed
Nommick,
Lrrcc1 and Ccdc61 are conserved effectors of multiciliated cell function.
2022,
Pubmed
,
Xenbase
Ossipova,
Planar polarization of Vangl2 in the vertebrate neural plate is controlled by Wnt and Myosin II signaling.
2015,
Pubmed
,
Xenbase
Park,
The planar cell-polarity gene stbm regulates cell behaviour and cell fate in vertebrate embryos.
2002,
Pubmed
,
Xenbase
Park,
Ciliogenesis defects in embryos lacking inturned or fuzzy function are associated with failure of planar cell polarity and Hedgehog signaling.
2006,
Pubmed
,
Xenbase
Puvirajesinghe,
Identification of p62/SQSTM1 as a component of non-canonical Wnt VANGL2-JNK signalling in breast cancer.
2016,
Pubmed
,
Xenbase
Rossmanith,
Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiation.
2011,
Pubmed
Sebbagh,
Insight into planar cell polarity.
2014,
Pubmed
Strutt,
Robust Asymmetric Localization of Planar Polarity Proteins Is Associated with Organization into Signalosome-like Domains of Variable Stoichiometry.
2016,
Pubmed
Taylor,
Van Gogh: a new Drosophila tissue polarity gene.
1998,
Pubmed
Torban,
Van Gogh-like2 (Strabismus) and its role in planar cell polarity and convergent extension in vertebrates.
2004,
Pubmed
,
Xenbase
Torban,
An expanding role of Vangl proteins in embryonic development.
2012,
Pubmed
Torban,
Genetic interaction between members of the Vangl family causes neural tube defects in mice.
2008,
Pubmed
Trulley,
Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2.
2019,
Pubmed
Van Damme,
N-terminal proteomics and ribosome profiling provide a comprehensive view of the alternative translation initiation landscape in mice and men.
2014,
Pubmed
Werner,
Using Xenopus skin to study cilia development and function.
2013,
Pubmed
,
Xenbase
Wolff,
Strabismus, a novel gene that regulates tissue polarity and cell fate decisions in Drosophila.
1998,
Pubmed
