Kosch TA , Torres-Sánchez M , Liedtke HC , Summers K , Yun MH , Crawford AJ , Maddock ST , Ahammed MS , Araújo VLN , Bertola LV , Bucciarelli GM , Carné A , Carneiro CM , Chan KO , Chen Y , Crottini A , da Silva JM , Denton RD , Dittrich C , Espregueira Themudo G , Farquharson KA , Forsdick NJ , Gilbert E , Che J , Katzenback BA , Kotharambath R , Levis NA , Márquez R , Mazepa G , Mulder KP , Müller H , O'Connell MJ , Orozco-terWengel P , Palomar G , Petzold A , Pfennig DW , Pfennig KS , Reichert MS , Robert J , Scherz MD , Siu-Ting K , Snead AA , Stöck M , Stuckert AMM , Stynoski JL , Tarvin RD , Wollenberg Valero KC , Amphibian Genomics Consortium .

	Fig. 1Estimated genome size across tetrapod classes in relation to sequenced genomes. A Mosaic plot representing the percentage of species with sequenced genomes as a proportion of the number of described species for each tetrapod class (Yes: % species with sequenced genome; No: % species without sequenced genome). B Combined box and density plot with raw data as points comparing genome size of species with sequenced genome (gray; genome sizes from NCBI genome assemblies) versus a subset of species without a sequenced genome (red; genome sizes from the Animal Genome Size Database) for each tetrapod class. The y-axis is log-transformed to facilitate visualization. Information about sequenced genomes and genome sizes was obtained from the NCBI Genome Browser, the Animal Genome Size database, and amphibian records from [12, 13]
	Fig. 2Estimated genome size across amphibian orders in relation to sequenced genomes. A Combined box and density plot with raw data as points showing genome size of species with sequenced genome (gray color; genome sizes from NCBI genome assemblies) versus a subset of species without available genome assembly (red color; genome sizes from the Animal Genome Size Database) for each amphibian order. The y-axis is logarithmic transformed to facilitate visualization. Information about sequenced genomes and genome sizes was obtained from the NCBI Genome Browser, the Animal Genome Size database [13], and amphibian records from [12]. B Amphibian phylogenetic tree was adapted from [17], which includes species with genome size estimates from Genomes on a Tree (GoaT) [18]. Branches are color coded to represent families without any genomic record (in red) and families with at least a representative genome sequenced (in gray). Bar plots around the phylogeny indicate relative genome sizes
	Fig. 3Main sequencing techniques applied to amphibian genomics studies. Yearly cumulative number of amphibian BioProjects split and color-coded by sequencing technique (DNA accessibility Sequencing includes ATAC-Seq and Mnase-Seq; Immunoprecipitation Sequencing includes: ChIP-Seq and RIP-Seq; Amplicon sequencing was included with Targeted-Capture Sequencing; Noncoding RNA Sequencing includes: miRNA-Seq and ncRNA-Seq). BioProject information was obtained from the NCBI Sequence Read Archive (SRA, accessed 1 March 2024)
	Fig. 4Amphibian Genomics Consortium (AGC) membership by country. Inset map showing the size of each country scaled by number of members in the AGC
	Fig. 5Sequencing competencies and identified challenges of the members of the Amphibian Genomics Consortium (AGC). A Representation of the contribution of the AGC survey quantitative questions to the first dimensions after computing a principal component analysis (PCA). Bioinformatic competencies and perceived challenges were grouped into dimensions one and two, respectively. B Scatter plot showing PCA scores for each AGC survey respondent. Respondent answers are coded by the qualitative question about funding success for amphibian genomics projects using shape; number of amphibian species of the respondent main affiliation country by size, and gross domestic expenditure on R&D (GERD) per capita of the respondent main affiliation country by gray-scale color coded. Information about the number of amphibian species per country was obtained from AmphibiaWeb. GERD per capita was calculated using information from the UNESCO and World Bank websites from the information about the most recent year for each country