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J Virol
2002 Dec 01;7624:12877-89. doi: 10.1128/jvi.76.24.12877-12889.2002.
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ICP27 interacts with the RNA export factor Aly/REF to direct herpes simplex virus type 1 intronless mRNAs to the TAP export pathway.
Chen IH
,
Sciabica KS
,
Sandri-Goldin RM
.
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Herpes simplex virus type 1 (HSV-1) protein ICP27 facilitates the export of viral intronless mRNAs. ICP27 shuttles between the nucleus and cytoplasm, which has been shown to require a leucine-rich nuclear export sequence (NES). ICP27 export was reported to be sensitive to the CRM1 inhibitor leptomycin B (LMB) in HSV-1-infected cells but not in Xenopus oocytes, where ICP27 interacts with the export factor Aly/REF to access the TAP export pathway. Here, we show that ICP27 interacts with Aly/REF in HSV-1-infected mammalian cells and that Aly/REF stimulates export of viral intronless RNAs but does not cross-link to these RNAs. During infection, Aly/REF was no longer associated with splicing factor SC35 but moved into structures that colocalized with ICP27, suggesting that ICP27 recruits Aly/REF from spliceosomes to viral intronless RNAs. Further, ICP27 was found to interact in vivo with TAP but not with CRM1. In vitro export assays showed that ICP27 export was not sensitive to LMB but was blocked by a dominant-negative TAP deletion mutant lacking the nucleoporin interaction domain. These data suggest that ICP27 uses the TAP pathway to export viral RNAs. Interestingly, the leucine-rich N-terminal sequence was required for efficient export, even though ICP27 export was LMB insensitive. Thus, this region is required for efficient ICP27 export but does not function as a CRM1-dependent NES.
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000, Pubmed,
Xenbase
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000,
Pubmed
,
Xenbase
Bear,
Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.
1999,
Pubmed
,
Xenbase
Braun,
Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.
2001,
Pubmed
,
Xenbase
Bryant,
Herpes simplex virus IE63 (ICP27) protein interacts with spliceosome-associated protein 145 and inhibits splicing prior to the first catalytic step.
2001,
Pubmed
Cullen,
Connections between the processing and nuclear export of mRNA: evidence for an export license?
2000,
Pubmed
Cullen,
Nuclear RNA export pathways.
2000,
Pubmed
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Friesen,
The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.
2001,
Pubmed
Gatfield,
The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
2001,
Pubmed
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Hardwicke,
The herpes simplex virus regulatory protein ICP27 contributes to the decrease in cellular mRNA levels during infection.
1994,
Pubmed
Hardy,
Herpes simplex virus inhibits host cell splicing, and regulatory protein ICP27 is required for this effect.
1994,
Pubmed
Herold,
TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.
2000,
Pubmed
Hibbard,
Arginine-rich regions succeeding the nuclear localization region of the herpes simplex virus type 1 regulatory protein ICP27 are required for efficient nuclear localization and late gene expression.
1995,
Pubmed
Kang,
The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.
1999,
Pubmed
Katahira,
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Kehlenbach,
Nucleocytoplasmic shuttling factors including Ran and CRM1 mediate nuclear export of NFAT In vitro.
1998,
Pubmed
Koffa,
Herpes simplex virus ICP27 protein provides viral mRNAs with access to the cellular mRNA export pathway.
2001,
Pubmed
,
Xenbase
Komeili,
New perspectives on nuclear transport.
2001,
Pubmed
Kudo,
Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1.
1998,
Pubmed
Le Hir,
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
2000,
Pubmed
,
Xenbase
Le Hir,
The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
2001,
Pubmed
,
Xenbase
Liu,
HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
1995,
Pubmed
Love,
Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.
1998,
Pubmed
Luo,
Splicing is required for rapid and efficient mRNA export in metazoans.
1999,
Pubmed
,
Xenbase
Luo,
Pre-mRNA splicing and mRNA export linked by direct interactions between UAP56 and Aly.
2001,
Pubmed
,
Xenbase
Mears,
Identification of nuclear and nucleolar localization signals in the herpes simplex virus regulatory protein ICP27.
1995,
Pubmed
,
Xenbase
Mears,
The RGG box motif of the herpes simplex virus ICP27 protein mediates an RNA-binding activity and determines in vivo methylation.
1996,
Pubmed
Mears,
The herpes simplex virus immediate-early protein ICP27 shuttles between nucleus and cytoplasm.
1998,
Pubmed
Meister,
Methylation of Sm proteins by a complex containing PRMT5 and the putative U snRNP assembly factor pICln.
2001,
Pubmed
,
Xenbase
Michael,
A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway.
1995,
Pubmed
Neubauer,
Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.
1998,
Pubmed
Neville,
The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae.
1999,
Pubmed
Otero,
Splicing-independent expression of the herpes simplex virus type 1 thymidine kinase gene is mediated by three cis-acting RNA subelements.
1998,
Pubmed
Phelan,
A herpes simplex virus type 1 immediate-early gene product, IE63, regulates small nuclear ribonucleoprotein distribution.
1993,
Pubmed
Phelan,
Herpes simplex virus type 1 immediate early protein IE63 shuttles between nuclear compartments and the cytoplasm.
1997,
Pubmed
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Rodrigues,
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
2001,
Pubmed
,
Xenbase
Sandri-Goldin,
The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-Sm antiserum, and the C terminus appears to be required for this interaction.
1996,
Pubmed
Sandri-Goldin,
ICP27 mediates HSV RNA export by shuttling through a leucine-rich nuclear export signal and binding viral intronless RNAs through an RGG motif.
1998,
Pubmed
Sandri-Goldin,
Interactions between a herpes simplex virus regulatory protein and cellular mRNA processing pathways.
1998,
Pubmed
Sandri-Goldin,
The C-terminal repressor region of herpes simplex virus type 1 ICP27 is required for the redistribution of small nuclear ribonucleoprotein particles and splicing factor SC35; however, these alterations are not sufficient to inhibit host cell splicing.
1995,
Pubmed
Santos-Rosa,
Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores.
1998,
Pubmed
Schmitt,
In vitro analysis of nuclear transport mediated by the C-terminal shuttle domain of Tap.
2001,
Pubmed
Segref,
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
1997,
Pubmed
Shen,
Arginine methylation facilitates the nuclear export of hnRNP proteins.
1998,
Pubmed
Smith,
Evidence that the herpes simplex virus immediate early protein ICP27 acts post-transcriptionally during infection to regulate gene expression.
1992,
Pubmed
Soliman,
Shuttling of the herpes simplex virus type 1 regulatory protein ICP27 between the nucleus and cytoplasm mediates the expression of late proteins.
1997,
Pubmed
Soliman,
Herpesvirus mRNAs are sorted for export via Crm1-dependent and -independent pathways.
2000,
Pubmed
Soliman,
Identification of an export control sequence and a requirement for the KH domains in ICP27 from herpes simplex virus type 1.
2000,
Pubmed
Strässer,
Splicing factor Sub2p is required for nuclear mRNA export through its interaction with Yra1p.
2001,
Pubmed
Strässer,
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.
2000,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Tan,
The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.
2000,
Pubmed
Wiegand,
Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes.
2002,
Pubmed
Zenklusen,
Nuclear export of mRNA.
2001,
Pubmed
Zenklusen,
The yeast hnRNP-Like proteins Yra1p and Yra2p participate in mRNA export through interaction with Mex67p.
2001,
Pubmed
Zhi,
Self-interaction of the herpes simplex virus type 1 regulatory protein ICP27.
1999,
Pubmed
Zhi,
Analysis of the phosphorylation sites of herpes simplex virus type 1 regulatory protein ICP27.
1999,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
