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RNA
2002 Oct 01;810:1267-79. doi: 10.1017/s1355838202029059.
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Inhibition of protein synthesis by aminoglycoside-arginine conjugates.
Carriere M
,
Vijayabaskar V
,
Applefield D
,
Harvey I
,
Garneau P
,
Lorsch J
,
Lapidot A
,
Pelletier J
.
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Inhibition of translation by small molecule ligands has proven to be a useful tool for understanding this complex cellular mechanism, as well as providing drugs of significant medical importance. Many small molecule ligands inhibit translation by binding to RNA or RNA/protein components of the ribosomal subunits and usurping their function. A class of peptidomimetics [aminoglycoside-arginine conjugates (AAC)] has recently been designed to inhibit HIV TAR/tat interaction and in experiments aimed at assessing the inhibitory effects of AACs on TAR-containing transcripts, we found that AACs are general inhibitors of translation. Experiments reported herein aim at characterizing these novel properties of AACs. We find that AACs are inhibitors of eukaryotic and prokaryotic translation and exert their effects by blocking peptide chain elongation. Structure/activity relationship studies suggest that inhibition of translation by AACs is directly related to the number of arginine groups present on the aminoglycoside backbone and to the nature of the core aminoglycoside. AACs are therefore attractive tools for understanding and probing ribosome function.
Anderson,
Preparation of a cell-free protein-synthesizing system from wheat germ.
1983, Pubmed
Anderson,
Preparation of a cell-free protein-synthesizing system from wheat germ.
1983,
Pubmed
Bauer,
Overexpression of the eukaryotic translation initiation factor 4G (eIF4G-1) in squamous cell lung carcinoma.
2002,
Pubmed
Beckers,
Inhibition of cytoplasmic and organellar protein synthesis in Toxoplasma gondii. Implications for the target of macrolide antibiotics.
1995,
Pubmed
Böttger,
Structural basis for selectivity and toxicity of ribosomal antibiotics.
2001,
Pubmed
Brodersen,
The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit.
2000,
Pubmed
Carter,
Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics.
2000,
Pubmed
Chen,
Prokaryotic coupled transcription-translation.
1983,
Pubmed
Cheng,
Design of RNA-binding proteins and ligands.
2001,
Pubmed
Earnshaw,
Hairpin ribozyme cleavage catalyzed by aminoglycoside antibiotics and the polyamine spermine in the absence of metal ions.
1998,
Pubmed
Eckstein,
Guanylylimidodiphosphate and its interaction with amino acid polymerization factors.
1971,
Pubmed
Eubank,
Inhibition of bacterial RNase P by aminoglycoside-arginine conjugates.
2002,
Pubmed
Gold,
Diversity of oligonucleotide functions.
1995,
Pubmed
Harvey,
Forced engagement of a RNA/protein complex by a chemical inducer of dimerization to modulate gene expression.
2002,
Pubmed
Hausner,
The allosteric three-site model for the ribosomal elongation cycle. New insights into the inhibition mechanisms of aminoglycosides, thiostrepton, and viomycin.
1988,
Pubmed
Hoch,
Antibiotic inhibition of RNA catalysis: neomycin B binds to the catalytic core of the td group I intron displacing essential metal ions.
1998,
Pubmed
Jenne,
Rapid identification and characterization of hammerhead-ribozyme inhibitors using fluorescence-based technology.
2001,
Pubmed
Kurtz,
Fidelity of protein synthesis with chicken embryo mitochondrial and cytoplasmic ribosomes.
1974,
Pubmed
Litovchick,
Neomycin B-arginine conjugate, a novel HIV-1 Tat antagonist: synthesis and anti-HIV activities.
2001,
Pubmed
Litovchick,
Aminoglycoside-arginine conjugates that bind TAR RNA: synthesis, characterization, and antiviral activity.
2000,
Pubmed
Litovchick,
Arginine-aminoglycoside conjugates that bind to HIV transactivation responsive element RNA in vitro.
1999,
Pubmed
Lorsch,
Kinetic dissection of fundamental processes of eukaryotic translation initiation in vitro.
1999,
Pubmed
Mehta,
30S ribosomal subunit assembly is a target for inhibition by aminoglycosides in Escherichia coli.
2002,
Pubmed
Mei,
Discovery of selective, small-molecule inhibitors of RNA complexes--I. The Tat protein/TAR RNA complexes required for HIV-1 transcription.
1997,
Pubmed
Merrick,
Translation of exogenous mRNAs in reticulocyte lysates.
1983,
Pubmed
Mikkelsen,
Inhibition of RNase P RNA cleavage by aminoglycosides.
1999,
Pubmed
Nathan,
Detection of the proto-oncogene eIF4E in surgical margins may predict recurrence in head and neck cancer.
1997,
Pubmed
Noller,
Ribosomal RNA and translation.
1991,
Pubmed
Palacián,
Interaction of arginine with the ribosomal peptidyl transferase centre.
1979,
Pubmed
Palmer,
Mistranslation in a eucaryotic organism.
1978,
Pubmed
Pelletier,
Insertion mutagenesis to increase secondary structure within the 5' noncoding region of a eukaryotic mRNA reduces translational efficiency.
1985,
Pubmed
Pioletti,
Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.
2001,
Pubmed
Puglisi,
Role of RNA structure in arginine recognition of TAR RNA.
1993,
Pubmed
Puglisi,
Conformation of the TAR RNA-arginine complex by NMR spectroscopy.
1992,
Pubmed
Rogers,
Inhibition of the self-cleavage reaction of the human hepatitis delta virus ribozyme by antibiotics.
1996,
Pubmed
Schlünzen,
Structural basis for the interaction of antibiotics with the peptidyl transferase centre in eubacteria.
2001,
Pubmed
Sor,
Erythromycin and spiramycin resistance mutations of yeast mitochondria: nature of the rib2 locus in the large ribosomal RNA gene.
1984,
Pubmed
Stage,
Inhibition of the hammerhead ribozyme by neomycin.
1995,
Pubmed
Svitkin,
Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation.
2001,
Pubmed
Svitkin,
General RNA binding proteins render translation cap dependent.
1996,
Pubmed
Svitkin,
Complete translation of encephalomyocarditis virus RNA and faithful cleavage of virus-specific proteins in a cell-free system from Krebs-2 cells.
1978,
Pubmed
Szittner,
Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium.
1990,
Pubmed
Tao,
Specific binding of arginine to TAR RNA.
1992,
Pubmed
Tarbé,
Transcriptional profiling of cell lines derived from an orthotopic pancreatic tumor model reveals metastasis-associated genes.
2001,
Pubmed
von Ahsen,
Antibiotic inhibition of group I ribozyme function.
1991,
Pubmed
Walter,
Binding of tobramycin leads to conformational changes in yeast tRNA(Asp) and inhibition of aminoacylation.
2002,
Pubmed
Walter,
Aminoglycoside-RNA interactions.
1999,
Pubmed
Wilhelm,
Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells.
1978,
Pubmed
Wilhelm,
Aminoglycoside antibiotics and eukaryotic protein synthesis: structure--function relationships in the stimulation of misreading with a wheat embryo system.
1978,
Pubmed
Zapp,
Small molecules that selectively block RNA binding of HIV-1 Rev protein inhibit Rev function and viral production.
1993,
Pubmed
