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Proc Natl Acad Sci U S A
2002 Oct 01;9920:13238-42. doi: 10.1073/pnas.192445299.
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Expression of functional neurotransmitter receptors in Xenopus oocytes after injection of human brain membranes.
Miledi R
,
Eusebi F
,
Martínez-Torres A
,
Palma E
,
Trettel F
.
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The Xenopus oocyte is a very powerful tool for studies of the structure and function of membrane proteins, e.g., messenger RNA extracted from the brain and injected into oocytes leads to the synthesis and membrane incorporation of many types of functional receptors and ion channels, and membrane vesicles from Torpedo electroplaques injected into oocytes fuse with the oocyte membrane and cause the appearance of functional Torpedo acetylcholine receptors and Cl(-) channels. This approach was developed further to transplant already assembled neurotransmitter receptors from human brain cells to the plasma membrane of Xenopus oocytes. Membranes isolated from the temporal neocortex of a patient, operated for intractable epilepsy, were injected into oocytes and, within a few hours, the oocyte membrane acquired functional neurotransmitter receptors to gamma-aminobutyric acid, alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate, and glycine. These receptors were also expressed in the plasma membrane of oocytes injected with mRNA extracted from the temporal neocortex of the same patient. All of this makes the Xenopus oocyte a more useful model than it already is for studies of the structure and function of many human membrane proteins and opens the way to novel pathophysiological investigations of some human brain disorders.
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982, Pubmed,
Xenbase
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Barrantes,
Structural-functional correlates of the nicotinic acetylcholine receptor and its lipid microenvironment.
1993,
Pubmed
Demuro,
Antagonistic action of pitrazepin on human and rat GABA(A) receptors.
1999,
Pubmed
,
Xenbase
Giovannelli,
Tunicamycin increases desensitization of acetylcholine receptors in cultured mouse muscle cells.
1991,
Pubmed
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Marsal,
Incorporation of acetylcholine receptors and Cl- channels in Xenopus oocytes injected with Torpedo electroplaque membranes.
1995,
Pubmed
,
Xenbase
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Morales,
Incorporation of reconstituted acetylcholine receptors from Torpedo into the Xenopus oocyte membrane.
1995,
Pubmed
,
Xenbase
Palma,
Threonine-for-leucine mutation within domain M2 of the neuronal alpha(7) nicotinic receptor converts 5-hydroxytryptamine from antagonist to agonist.
1996,
Pubmed
,
Xenbase
Parker,
Actions of pentobarbital on rat brain receptors expressed in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Parker,
Responses to GABA, glycine and beta-alanine induced in Xenopus oocytes by messenger RNA from chick and rat brain.
1988,
Pubmed
,
Xenbase
Patneau,
Hippocampal neurons exhibit cyclothiazide-sensitive rapidly desensitizing responses to kainate.
1993,
Pubmed
Sigel,
Use of Xenopus oocytes for the functional expression of plasma membrane proteins.
1990,
Pubmed
,
Xenbase
Sigel,
The benzodiazepine binding site of GABAA receptors.
1997,
Pubmed
Sumikawa,
Partial purification and functional expression of brain mRNAs coding for neurotransmitter receptors and voltage-operated channels.
1984,
Pubmed
,
Xenbase
Sumikawa,
Assembly and N-glycosylation of all ACh receptor subunits are required for their efficient insertion into plasma membranes.
1989,
Pubmed
,
Xenbase
Walters,
Benzodiazepines act on GABAA receptors via two distinct and separable mechanisms.
2000,
Pubmed
,
Xenbase
Woodward,
Characterization of bicuculline/baclofen-insensitive (rho-like) gamma-aminobutyric acid receptors expressed in Xenopus oocytes. II. Pharmacology of gamma-aminobutyric acidA and gamma-aminobutyric acidB receptor agonists and antagonists.
1993,
Pubmed
,
Xenbase