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Kv1 potassium channels consist of pore-forming alpha subunits as well as auxiliary beta subunits. In heterologous systems, Kv1alpha subunits suffice for induction of voltage-dependent potassium current (I(Kv)). Although Kv1 channels can be expressed without auxiliary subunits in heterologous systems, coexpression with Kvbeta subunits has dramatic effects on surface expression and kinetic properties. Much less is known about the functional roles of Kvbeta subunits in vivo, despite their presence in the majority of native Kv1 channel complexes. We used an antisense approach to probe the contribution of Kvbeta2 subunits to native Kv1 channel function in embryonic myocytes. We compared the effects of antisense Kvbeta2 treatment on the whole cell I(Kv) to those produced by overexpression of a dominant-negative Kv1alpha subunit. The reductions in the maximal potassium conductance produced by antisense Kvbeta2 treatment and elimination of Kv1alpha subunit function were not significantly different from each other. In addition, simultaneous elimination of Kv1alpha and Kvbeta2 subunit function resulted in no further reduction of the maximal conductance. The Kv channel complexes targeted by Kvbeta2 and/or Kv1alpha subunit elimination contributed to action potential repolarization because elimination of either or both subunits led to increases in the duration of the action potential. As for potassium conductance, the effects of elimination of both alpha and beta subunits on the duration of the action potential were not additive. Taken together, the results suggest that Kv1 potassium channel complexes in vivo have a strong requirement for both alpha and beta subunits.
Barry,
Functional knockout of the transient outward current, long-QT syndrome, and cardiac remodeling in mice expressing a dominant-negative Kv4 alpha subunit.
1998, Pubmed
Barry,
Functional knockout of the transient outward current, long-QT syndrome, and cardiac remodeling in mice expressing a dominant-negative Kv4 alpha subunit.
1998,
Pubmed
Blaine,
Kv2 channels form delayed-rectifier potassium channels in situ.
2001,
Pubmed
,
Xenbase
Brenner,
Vasoregulation by the beta1 subunit of the calcium-activated potassium channel.
2000,
Pubmed
Burger,
Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development.
1996,
Pubmed
,
Xenbase
Carl,
Inhibition of neural crest migration in Xenopus using antisense slug RNA.
1999,
Pubmed
,
Xenbase
Chen,
Sh and eag K(+) channel subunit interaction in frog oocytes depends on level and time of expression.
2000,
Pubmed
,
Xenbase
Chen,
Heteromultimeric interactions among K+ channel subunits from Shaker and eag families in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Chouinard,
A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.
1995,
Pubmed
,
Xenbase
DeCino,
Development and subsequent neural tube effects on the excitability of cultured Xenopus myocytes.
1985,
Pubmed
,
Xenbase
Espinosa,
Alcohol hypersensitivity, increased locomotion, and spontaneous myoclonus in mice lacking the potassium channels Kv3.1 and Kv3.3.
2001,
Pubmed
Feng,
Antisense oligodeoxynucleotides directed against Kv1.5 mRNA specifically inhibit ultrarapid delayed rectifier K+ current in cultured adult human atrial myocytes.
1997,
Pubmed
Ferreiro,
XASH1, a Xenopus homolog of achaete-scute: a proneural gene in anterior regions of the vertebrate CNS.
1993,
Pubmed
,
Xenbase
Fry,
Cloning and expression of three K+ channel cDNAs from Xenopus muscle.
2001,
Pubmed
,
Xenbase
Giese,
Reduced K+ channel inactivation, spike broadening, and after-hyperpolarization in Kvbeta1.1-deficient mice with impaired learning.
1998,
Pubmed
Gulbis,
Structure of a voltage-dependent K+ channel beta subunit.
1999,
Pubmed
Guo,
Functional consequences of elimination of i(to,f) and i(to,s): early afterdepolarizations, atrioventricular block, and ventricular arrhythmias in mice lacking Kv1.4 and expressing a dominant-negative Kv4 alpha subunit.
2000,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Jones,
Overexpression of a potassium channel gene perturbs neural differentiation.
1994,
Pubmed
,
Xenbase
Kil,
Inhibition of cranial neural crest adhesion in vitro and migration in vivo using integrin antisense oligonucleotides.
1996,
Pubmed
Lazaroff,
Xenopus embryonic spinal neurons express potassium channel Kvbeta subunits.
1999,
Pubmed
,
Xenbase
Listerud,
Functional contribution of neuronal AChR subunits revealed by antisense oligonucleotides.
1991,
Pubmed
London,
Targeted replacement of KV1.5 in the mouse leads to loss of the 4-aminopyridine-sensitive component of I(K,slow) and resistance to drug-induced qt prolongation.
2001,
Pubmed
McCormack,
Shaker K+ channel beta subunits belong to an NAD(P)H-dependent oxidoreductase superfamily.
1994,
Pubmed
Nakahira,
Selective interaction of voltage-gated K+ channel beta-subunits with alpha-subunits.
1996,
Pubmed
Plüger,
Mice with disrupted BK channel beta1 subunit gene feature abnormal Ca(2+) spark/STOC coupling and elevated blood pressure.
2000,
Pubmed
Pongs,
Functional and molecular aspects of voltage-gated K+ channel beta subunits.
1999,
Pubmed
,
Xenbase
Rétaux,
Engrailed, retinotectal targeting, and axonal patterning in the midbrain during Xenopus development: an antisense study.
1996,
Pubmed
,
Xenbase
Rhodes,
Voltage-gated K+ channel beta subunits: expression and distribution of Kv beta 1 and Kv beta 2 in adult rat brain.
1996,
Pubmed
Ribera,
Developmental regulation of potassium channels and the impact on neuronal differentiation.
1992,
Pubmed
Ribera,
Differentiation of delayed rectifier potassium current in embryonic amphibian myocytes.
1991,
Pubmed
,
Xenbase
Ribera,
A critical period of transcription required for differentiation of the action potential of spinal neurons.
1989,
Pubmed
,
Xenbase
Ribera,
Primary sensory neurons express a Shaker-like potassium channel gene.
1993,
Pubmed
,
Xenbase
Ribera,
Homogeneous development of electrical excitability via heterogeneous ion channel expression.
1996,
Pubmed
,
Xenbase
Roy,
Manipulation of the delayed rectifier Kv1.5 potassium channel in glial cells by antisense oligodeoxynucleotides.
1996,
Pubmed
Shamotienko,
Subunit combinations defined for K+ channel Kv1 subtypes in synaptic membranes from bovine brain.
1997,
Pubmed
Spitzer,
The development of the action potential mechanism of amphibian neurons isolated in culture.
1976,
Pubmed
,
Xenbase
Tang,
Shaker and ether-à-go-go K+ channel subunits fail to coassemble in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Trimmer,
Regulation of ion channel expression by cytoplasmic subunits.
1998,
Pubmed
Vincent,
Antisense suppression of potassium channel expression demonstrates its role in maturation of the action potential.
2000,
Pubmed
,
Xenbase
Wang,
In vivo functional role of the Drosophila hyperkinetic beta subunit in gating and inactivation of Shaker K+ channels.
1996,
Pubmed
Wilson,
Interaction of the K channel beta subunit, Hyperkinetic, with eag family members.
1998,
Pubmed
,
Xenbase
Woolf,
Specificity of antisense oligonucleotides in vivo.
1992,
Pubmed
,
Xenbase
Xu,
Distinct functional stoichiometry of potassium channel beta subunits.
1998,
Pubmed
Xu,
Elimination of the transient outward current and action potential prolongation in mouse atrial myocytes expressing a dominant negative Kv4 alpha subunit.
1999,
Pubmed
Yang,
Kvbeta subunits increase expression of Kv4.3 channels by interacting with their C termini.
2001,
Pubmed
Yao,
Auxiliary Hyperkinetic beta subunit of K+ channels: regulation of firing properties and K+ currents in Drosophila neurons.
1999,
Pubmed
Yu,
Uptake of antisense oligonucleotides and functional block of acetylcholine receptor subunit gene expression in primary embryonic neurons.
1993,
Pubmed
