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RNA
2002 Mar 01;83:345-56. doi: 10.1017/s1355838202027759.
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An element in the 3' untranslated region of human LINE-1 retrotransposon mRNA binds NXF1(TAP) and can function as a nuclear export element.
Lindtner S
,
Felber BK
,
Kjems J
.
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Export of unspliced mRNA to the cytoplasm is required for the replication of all retroviruses. In simian type D retroviruses, the RNA export is mediated by the constitutive transport element (CTE) that binds the cellular nuclear export factor 1, NXF1(TAP). To search for potential cellular RNA substrates for NXF1, we have set up an in vitro selection procedure, using an RNA library expressed from total human genomic DNA. A sequence that was isolated most frequently as independent clones exhibits extensive homology to the 3' untranslated region of expressed LINE1 (L1) retrotransposons. This region, termed L1-NXF1 binding element (L1-NBE) bears no structural resemblance to the viral CTE, but binds NXF1 as strongly as CTE, based on gel mobility shift competition assays. A deletion analysis of the NXF1 protein reveals that CTE and L1-NBE have different, but overlapping, binding domains on NXF1. Placed in an intron, L1-NBE is capable of mediating nuclear export of lariat RNA species in Xenopus laevis oocytes and of an unspliced HIV-1 derived RNA in human 293 cells, suggesting that it may function as a nuclear export element for the intronless L1 mRNA.
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000, Pubmed,
Xenbase
Bachi,
The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates.
2000,
Pubmed
,
Xenbase
Bear,
Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs.
1999,
Pubmed
,
Xenbase
Black,
Human T-cell leukemia virus type II Rex binding and activity require an intact splice donor site and a specific RNA secondary structure.
1991,
Pubmed
Braun,
TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus.
1999,
Pubmed
,
Xenbase
Braun,
Overexpression of TAP/p15 heterodimers bypasses nuclear retention and stimulates nuclear mRNA export.
2001,
Pubmed
,
Xenbase
Bray,
A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent.
1994,
Pubmed
Burd,
Conserved structures and diversity of functions of RNA-binding proteins.
1994,
Pubmed
Donello,
Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element.
1998,
Pubmed
Ernst,
A structured retroviral RNA element that mediates nucleocytoplasmic export of intron-containing RNA.
1997,
Pubmed
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Guzik,
NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells.
2001,
Pubmed
Herold,
TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.
2000,
Pubmed
Hope,
Steroid-receptor fusion of the human immunodeficiency virus type 1 Rev transactivator: mapping cryptic functions of the arginine-rich motif.
1990,
Pubmed
Hope,
trans-dominant inhibition of human immunodeficiency virus type 1 Rev occurs through formation of inactive protein complexes.
1992,
Pubmed
Huang,
Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.
1999,
Pubmed
Huang,
Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA.
2001,
Pubmed
,
Xenbase
Huang,
The mouse histone H2a gene contains a small element that facilitates cytoplasmic accumulation of intronless gene transcripts and of unspliced HIV-1-related mRNAs.
1997,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kang,
Analysis of the RNA binding specificity of the human tap protein, a constitutive transport element-specific nuclear RNA export factor.
1999,
Pubmed
Kang,
The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences.
1999,
Pubmed
Kang,
Analysis of cellular factors that mediate nuclear export of RNAs bearing the Mason-Pfizer monkey virus constitutive transport element.
2000,
Pubmed
Katahira,
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
1999,
Pubmed
Kataoka,
Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm.
2000,
Pubmed
,
Xenbase
Kazazian,
The impact of L1 retrotransposons on the human genome.
1998,
Pubmed
Kazazian,
Haemophilia A resulting from de novo insertion of L1 sequences represents a novel mechanism for mutation in man.
1988,
Pubmed
Kjems,
The basic domain of Rev from human immunodeficiency virus type 1 specifically blocks the entry of U4/U6.U5 small nuclear ribonucleoprotein in spliceosome assembly.
1993,
Pubmed
Kjems,
Rev protein and its cellular partners.
2000,
Pubmed
Kobe,
The leucine-rich repeat: a versatile binding motif.
1994,
Pubmed
Kurose,
RNA polymerase III dependence of the human L1 promoter and possible participation of the RNA polymerase II factor YY1 in the RNA polymerase III transcription system.
1995,
Pubmed
Lander,
Initial sequencing and analysis of the human genome.
2001,
Pubmed
Le Hir,
The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
2000,
Pubmed
,
Xenbase
Liker,
The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain.
2000,
Pubmed
Liu,
HnRNP L binds a cis-acting RNA sequence element that enables intron-dependent gene expression.
1995,
Pubmed
Magin,
cORF and RcRE, the Rev/Rex and RRE/RxRE homologues of the human endogenous retrovirus family HTDV/HERV-K.
1999,
Pubmed
Moran,
High frequency retrotransposition in cultured mammalian cells.
1996,
Pubmed
Moran,
Human L1 retrotransposition: insights and peculiarities learned from a cultured cell retrotransposition assay.
1999,
Pubmed
Morse,
Insertional mutagenesis of the myc locus by a LINE-1 sequence in a human breast carcinoma.
1988,
Pubmed
Narita,
Insertion of a 5' truncated L1 element into the 3' end of exon 44 of the dystrophin gene resulted in skipping of the exon during splicing in a case of Duchenne muscular dystrophy.
1993,
Pubmed
Ogert,
Avian retroviral RNA element promotes unspliced RNA accumulation in the cytoplasm.
1996,
Pubmed
Otero,
Splicing-independent expression of the herpes simplex virus type 1 thymidine kinase gene is mediated by three cis-acting RNA subelements.
1998,
Pubmed
Pasquinelli,
The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway.
1997,
Pubmed
,
Xenbase
Pollard,
The HIV-1 Rev protein.
1998,
Pubmed
Rodrigues,
REF proteins mediate the export of spliced and unspliced mRNAs from the nucleus.
2001,
Pubmed
,
Xenbase
Saavedra,
The simian retrovirus-1 constitutive transport element, unlike the HIV-1 RRE, uses factors required for cellular mRNA export.
1997,
Pubmed
,
Xenbase
Sassaman,
Many human L1 elements are capable of retrotransposition.
1997,
Pubmed
Segref,
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
1997,
Pubmed
Singer,
Libraries for genomic SELEX.
1997,
Pubmed
Smit,
The origin of interspersed repeats in the human genome.
1996,
Pubmed
Strässer,
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.
2000,
Pubmed
Stutz,
REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export.
2000,
Pubmed
,
Xenbase
Tabernero,
Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1.
1997,
Pubmed
Tabernero,
The posttranscriptional control element of the simian retrovirus type 1 forms an extensive RNA secondary structure necessary for its function.
1996,
Pubmed
Tan,
The mRNA export in Caenorhabditis elegans is mediated by Ce-NXF-1, an ortholog of human TAP/NXF and Saccharomyces cerevisiae Mex67p.
2000,
Pubmed
Vankan,
Domains of U4 and U6 snRNAs required for snRNP assembly and splicing complementation in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Varani,
RNA recognition by RNP proteins during RNA processing.
1998,
Pubmed
Yang,
An ancient family of human endogenous retroviruses encodes a functional homolog of the HIV-1 Rev protein.
1999,
Pubmed
Yang,
Structural and functional analysis of the avian leukemia virus constitutive transport element.
1999,
Pubmed
Zhou,
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans.
2000,
Pubmed
Zolotukhin,
Retroviral constitutive transport element evolved from cellular TAP(NXF1)-binding sequences.
2001,
Pubmed
Zolotukhin,
Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes.
1994,
Pubmed
