Georges SA et al. (2002), p300-mediated tax transactivation from recombin...

XB-ART-7984

Mol Cell Biol 2002 Jan 01;221:127-37. doi: 10.1128/MCB.22.1.127-137.2002.

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p300-mediated tax transactivation from recombinant chromatin: histone tail deletion mimics coactivator function.

Georges SA , Kraus WL , Luger K , Nyborg JK , Laybourn PJ .

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Efficient transcription of the human T-cell leukemia virus type 1 (HTLV-1) genome requires Tax, a virally encoded oncogenic transcription factor, in complex with the cellular transcription factor CREB and the coactivators p300/CBP. To examine Tax transactivation in vitro, we used a chromatin assembly system that included recombinant core histones. The addition of Tax, CREB, and p300 to the HTLV-1 promoter assembled into chromatin activated transcription several hundredfold. Chromatin templates selectively lacking amino-terminal histone tails demonstrated enhanced transcriptional activation by Tax and CREB, with significantly reduced dependence on p300 and acetyl coenzyme A (acetyl-CoA). Interestingly, Tax/CREB activation from the tailless chromatin templates retained a substantial requirement for acetyl-CoA, indicating a role for acetyl-CoA beyond histone acetylation. These data indicate that during Tax transcriptional activation, the amino-terminal histone tails are the major targets of p300 and that tail deletion and acetylation are functionally equivalent.

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Species referenced: Xenopus laevis
Genes referenced: creb1 crebbp ep300 nr2e1

References [+] :

Adya, Distinct regions in human T-cell lymphotropic virus type I tax mediate interactions with activator protein CREB and basal transcription factors. 1995, Pubmed

Adya, Distinct regions in human T-cell lymphotropic virus type I tax mediate interactions with activator protein CREB and basal transcription factors. 1995, Pubmed
Anderson, Identification of poly(ADP-ribose) polymerase as a transcriptional coactivator of the human T-cell leukemia virus type 1 Tax protein. 2000, Pubmed
Ausio, Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome. 1989, Pubmed
Ausio, Histone hyperacetylation: its effects on nucleosome conformation and stability. 1986, Pubmed
Bannister, CBP-induced stimulation of c-Fos activity is abrogated by E1A. 1995, Pubmed
Bannister, The CBP co-activator is a histone acetyltransferase. , Pubmed
Böhm, Proteases as structural probes for chromatin: the domain structure of histones. 1984, Pubmed
Cann, Identification of the gene responsible for human T-cell leukaemia virus transcriptional regulation. , Pubmed
Carruthers, The core histone N termini function independently of linker histones during chromatin condensation. 2000, Pubmed
Chen, HATs on and beyond chromatin. 2001, Pubmed
Clapier, Critical role for the histone H4 N terminus in nucleosome remodeling by ISWI. 2001, Pubmed , Xenbase
Dallas, Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes. 1997, Pubmed
Dilworth, ATP-driven chromatin remodeling activity and histone acetyltransferases act sequentially during transactivation by RAR/RXR In vitro. 2000, Pubmed
Felber, The pX protein of HTLV-I is a transcriptional activator of its long terminal repeats. 1985, Pubmed
Franklin, Transactivation by the human T-cell leukemia virus Tax protein is mediated through enhanced binding of activating transcription factor-2 (ATF-2) ATF-2 response and cAMP element-binding protein (CREB). 1993, Pubmed
Galasinski, Acetyl coenzyme A stimulates RNA polymerase II transcription and promoter binding by transcription factor IID in the absence of histones. 2000, Pubmed
Galvin, Multiple mechanisms of transcriptional repression by YY1. 1997, Pubmed
Garcia-Ramirez, Modulation of chromatin folding by histone acetylation. 1995, Pubmed
Giebler, Anchoring of CREB binding protein to the human T-cell leukemia virus type 1 promoter: a molecular mechanism of Tax transactivation. 1997, Pubmed
Giordano, p300 and CBP: partners for life and death. 1999, Pubmed
Godde, The amino-terminal tails of the core histones and the translational position of the TATA box determine TBP/TFIIA association with nucleosomal DNA. 1995, Pubmed , Xenbase
Goodman, CBP/p300 in cell growth, transformation, and development. 2000, Pubmed
Goren, The amino terminus of Tax is required for interaction with the cyclic AMP response element binding protein. 1995, Pubmed
Hansen, A role for histones H2A/H2B in chromatin folding and transcriptional repression. 1994, Pubmed , Xenbase
Hansen, Structure and function of the core histone N-termini: more than meets the eye. 1998, Pubmed
Hansen, Influence of chromatin folding on transcription initiation and elongation by RNA polymerase III. 1992, Pubmed , Xenbase
Harrod, An exposed KID-like domain in human T-cell lymphotropic virus type 1 Tax is responsible for the recruitment of coactivators CBP/p300. 1998, Pubmed
Hayes, Histone contributions to the structure of DNA in the nucleosome. 1991, Pubmed , Xenbase
Imhof, Acetylation of general transcription factors by histone acetyltransferases. 1997, Pubmed
Ito, ACF consists of two subunits, Acf1 and ISWI, that function cooperatively in the ATP-dependent catalysis of chromatin assembly. 1999, Pubmed
Ito, p300-mediated acetylation facilitates the transfer of histone H2A-H2B dimers from nucleosomes to a histone chaperone. 2000, Pubmed
Ito, Drosophila NAP-1 is a core histone chaperone that functions in ATP-facilitated assembly of regularly spaced nucleosomal arrays. 1996, Pubmed
Ito, ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor. 1997, Pubmed
Jiang, PCAF interacts with tax and stimulates tax transactivation in a histone acetyltransferase-independent manner. 1999, Pubmed
Jiang, Transcriptional analysis of chromatin assembled with purified ACF and dNAP1 reveals that acetyl-CoA is required for preinitiation complex assembly. 2000, Pubmed
Kashanchi, The coactivator CBP stimulates human T-cell lymphotrophic virus type I Tax transactivation in vitro. 1998, Pubmed
Kim, Efficient recruitment of TFIIB and CBP-RNA polymerase II holoenzyme by an interferon-beta enhanceosome in vitro. 1998, Pubmed
Kimzey, Specific regions of contact between human T-cell leukemia virus type I Tax protein and DNA identified by photocross-linking. 1998, Pubmed
Kiyokawa, p27x-III and p21x-III, proteins encoded by the pX sequence of human T-cell leukemia virus type I. 1985, Pubmed
Kraus, p300 and estrogen receptor cooperatively activate transcription via differential enhancement of initiation and reinitiation. 1998, Pubmed
Kraus, Biochemical analysis of distinct activation functions in p300 that enhance transcription initiation with chromatin templates. 1999, Pubmed
Kundu, Activator-dependent transcription from chromatin in vitro involving targeted histone acetylation by p300. 2000, Pubmed
Kwok, Nuclear protein CBP is a coactivator for the transcription factor CREB. 1994, Pubmed
Kwok, Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP. 1996, Pubmed
Lee, A positive role for histone acetylation in transcription factor access to nucleosomal DNA. 1993, Pubmed , Xenbase
Lemasson, Human T-cell leukemia virus type I tax repression of p73beta is mediated through competition for the C/H1 domain of CBP. 2001, Pubmed
Lenzmeier, The tax protein-DNA interaction is essential for HTLV-I transactivation in vitro. 1999, Pubmed
Lenzmeier, Human T-cell leukemia virus type 1 Tax requires direct access to DNA for recruitment of CREB binding protein to the viral promoter. 1998, Pubmed
Li, p300 stimulates transcription instigated by ligand-bound thyroid hormone receptor at a step subsequent to chromatin disruption. 1999, Pubmed , Xenbase
Libertini, Histone hyperacetylation. Its effects on nucleosome core particle transitions. 1988, Pubmed
Luger, Preparation of nucleosome core particle from recombinant histones. 1999, Pubmed , Xenbase
Luger, Characterization of nucleosome core particles containing histone proteins made in bacteria. 1997, Pubmed , Xenbase
Lundblad, The human T-cell leukemia virus-1 transcriptional activator Tax enhances cAMP-responsive element-binding protein (CREB) binding activity through interactions with the DNA minor groove. 1998, Pubmed
Manning, p300 forms a stable, template-committed complex with chromatin: role for the bromodomain. 2001, Pubmed
Mayall, Distinct roles for P-CREB and LEF-1 in TCR alpha enhancer assembly and activation on chromatin templates in vitro. 1997, Pubmed
McGhee, Histone hyperacetylation has little effect on the higher order folding of chromatin. 1983, Pubmed
Morales, Role of histone N-terminal tails and their acetylation in nucleosome dynamics. 2000, Pubmed
Näär, Chromatin, TAFs, and a novel multiprotein coactivator are required for synergistic activation by Sp1 and SREBP-1a in vitro. 1998, Pubmed
Neish, Factors associated with the mammalian RNA polymerase II holoenzyme. 1998, Pubmed
Ogryzko, The transcriptional coactivators p300 and CBP are histone acetyltransferases. 1996, Pubmed
Oliva, Histone hyperacetylation can induce unfolding of the nucleosome core particle. 1990, Pubmed
Parekh, Virus infection leads to localized hyperacetylation of histones H3 and H4 at the IFN-beta promoter. 1999, Pubmed
Pazin, NF-kappa B-mediated chromatin reconfiguration and transcriptional activation of the HIV-1 enhancer in vitro. 1996, Pubmed
Pelletier, Competitive recruitment of CBP and Rb-HDAC regulates UBF acetylation and ribosomal transcription. 2000, Pubmed , Xenbase
Polesskaya, Interaction between acetylated MyoD and the bromodomain of CBP and/or p300. 2001, Pubmed
Protacio, Effects of histone tail domains on the rate of transcriptional elongation through a nucleosome. 2000, Pubmed
Puri, p300 is required for MyoD-dependent cell cycle arrest and muscle-specific gene transcription. 1997, Pubmed
Scoggin, The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation. 2001, Pubmed
Seiki, Direct evidence that p40x of human T-cell leukemia virus type I is a trans-acting transcriptional activator. 1986, Pubmed
Sheridan, Activation of the HIV-1 enhancer by the LEF-1 HMG protein on nucleosome-assembled DNA in vitro. 1995, Pubmed
Simpson, Histone-DNA interactions in chromatin core particles. 1978, Pubmed
Sodroski, A transcriptional activator protein encoded by the x-lor region of the human T-cell leukemia virus. 1985, Pubmed
Tse, Hybrid trypsinized nucleosomal arrays: identification of multiple functional roles of the H2A/H2B and H3/H4 N-termini in chromatin fiber compaction. 1997, Pubmed
Tse, Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III. 1998, Pubmed , Xenbase
Van Orden, Insight into the tumor suppressor function of CBP through the viral oncoprotein tax. 2000, Pubmed
Vettese-Dadey, Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro. 1996, Pubmed
Whitlock, Folding of DNA by histones which lack their NH2-terminal regions. 1978, Pubmed
Yie, Mechanism by which the IFN-beta enhanceosome activates transcription. 1999, Pubmed
Zhang, Site-specific acetylation by p300 or CREB binding protein regulates erythroid Krüppel-like factor transcriptional activity via its interaction with the SWI-SNF complex. 2001, Pubmed
Zhao, Interaction of the human T-cell lymphotrophic virus type I (HTLV-I) transcriptional activator Tax with cellular factors that bind specifically to the 21-base-pair repeats in the HTLV-I enhancer. 1991, Pubmed
Zhao, Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction. 1992, Pubmed