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Mol Biol Cell
2001 Nov 01;1211:3527-37. doi: 10.1091/mbc.12.11.3527.
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Cell cycle-dependent expression and nucleolar localization of hCAP-H.
Cabello OA
,
Eliseeva E
,
He WG
,
Youssoufian H
,
Plon SE
,
Brinkley BR
,
Belmont JW
.
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Condensin is a conserved 13S heteropentamer composed of two nonidentical structural maintenance of chromosome (SMC) family proteins, in Xenopus XCAP-C and XCAP-E, and three regulatory subunits, XCAP-D2, XCAP-G, and XCAP-H. Both biochemical and genetic analyses have demonstrated an essential role for the 13S condensin complex in mitotic chromosome condensation. Further, a potential requirement for condensin in completion of chromatid arm separation in early anaphase is demonstrated by the mutational phenotypes of the Drosophila homologues of XCAP-H, barren and XCAP-C, DmSMC4. In this study we have investigated the expression and subcellular distribution of hCAP-H, the human homolog of XCAP-H, in order to better understand its cellular functions. Transcription of hCAP-H was restricted to proliferating cells with highest expression during the G(2) phase of the cell cycle. In contrast, cellular hCAP-H protein levels were constant throughout the cell cycle. hCAP-H was found to be associated with mitotic chromosomes exhibiting a nonuniform but symmetric distribution along sister chromatids. The symmetry of hCAP-H association with sister chromatids suggests that there are sequence-dependent domains of condensin aggregation. During interphase hCAP-H, -C, and -E, have distinct punctate nucleolar localization, suggesting that condensin may associate with and modulate the conformation and function of rDNA. hCAP-H association with condensed chromatin was not observed in the early phase of chromosome condensation when histone H3 phosphorylation has already taken place. This finding is consistent with the hypothesis that histone H3 phosphorylation precedes condensin-mediated condensation.
Austin,
Expression, domain structure, and enzymatic properties of an active recombinant human DNA topoisomerase II beta.
1995, Pubmed
Austin,
Expression, domain structure, and enzymatic properties of an active recombinant human DNA topoisomerase II beta.
1995,
Pubmed
Bachant,
Mitotic treasures in the nucleolus.
1999,
Pubmed
Bhat,
Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with Topoisomerase II.
1996,
Pubmed
Blat,
Cohesins bind to preferential sites along yeast chromosome III, with differential regulation along arms versus the centric region.
1999,
Pubmed
Cabello,
Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2.
1997,
Pubmed
Cho,
A genome-wide transcriptional analysis of the mitotic cell cycle.
1998,
Pubmed
Freeman,
The condensin complex governs chromosome condensation and mitotic transmission of rDNA.
2000,
Pubmed
Giet,
Drosophila aurora B kinase is required for histone H3 phosphorylation and condensin recruitment during chromosome condensation and to organize the central spindle during cytokinesis.
2001,
Pubmed
Guacci,
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.
1997,
Pubmed
Guacci,
Structure and function of chromosomes in mitosis of budding yeast.
1993,
Pubmed
Hendzel,
Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.
1997,
Pubmed
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
SMC-mediated chromosome mechanics: a conserved scheme from bacteria to vertebrates?
1999,
Pubmed
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Kimura,
Phosphorylation and activation of 13S condensin by Cdc2 in vitro.
1998,
Pubmed
,
Xenbase
Kimura,
13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation.
1999,
Pubmed
,
Xenbase
Kimura,
Chromosome condensation by a human condensin complex in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Kimura,
ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation.
1997,
Pubmed
,
Xenbase
Kimura,
Dual roles of the 11S regulatory subcomplex in condensin functions.
2000,
Pubmed
,
Xenbase
Lavoie,
Mitotic chromosome condensation requires Brn1p, the yeast homologue of Barren.
2000,
Pubmed
,
Xenbase
Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase
Michaelis,
Cohesins: chromosomal proteins that prevent premature separation of sister chromatids.
1997,
Pubmed
Moroi,
Autoantibody to centromere (kinetochore) in scleroderma sera.
1980,
Pubmed
Nomura,
Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.
1994,
Pubmed
Ouspenski,
Chromosome condensation factor Brn1p is required for chromatid separation in mitosis.
2000,
Pubmed
,
Xenbase
Saka,
Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.
1994,
Pubmed
Sauvé,
Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic chromosome condensation.
1999,
Pubmed
Schmiesing,
Identification of two distinct human SMC protein complexes involved in mitotic chromosome dynamics.
1998,
Pubmed
,
Xenbase
Schmiesing,
A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation.
2000,
Pubmed
,
Xenbase
Steffensen,
A role for Drosophila SMC4 in the resolution of sister chromatids in mitosis.
2001,
Pubmed
Strunnikov,
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.
1995,
Pubmed
Sutani,
Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4.
1999,
Pubmed
,
Xenbase
Sutani,
DNA renaturation activity of the SMC complex implicated in chromosome condensation.
1997,
Pubmed
Tanaka,
Identification of cohesin association sites at centromeres and along chromosome arms.
1999,
Pubmed
Wei,
Phosphorylation of histone H3 is required for proper chromosome condensation and segregation.
1999,
Pubmed
