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Glycoconj J
2001 Mar 01;183:225-30. doi: 10.1023/a:1012400623096.
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Galactosides and sialylgalactosides in O-linked oligosaccharides of the primordial germ cells in Xenopus embryos.
Alonso E
,
Sáez FJ
,
Madrid JF
,
Hernández F
.
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The primordial germ cells (PGCs) are covered by surface glycoconjugates; some of them, like galactose residues recognized by peanut agglutinin (PNA), have been reported to be implicated in the PGC migration process. The aim of this work was the characterization of galactosides and sialylgalactosides in N- and O-linked oligosaccharides of Xenopus PGCs. Galactose(Gal)- and sialic acid(Neu5Ac)-binding lectin cytochemistry, in combination with chemical and enzymatic deglycosylation methods, were used. PGCs were slightly labeled with PNA, RCA-I and BSI-B4, which suggests the presence of the sequences Gal(beta1,4)GlcNAc and Gal(alpha1,3)Gal. Moreover, there was no labeling when beta-elimination pre-treatment was performed, suggesting that galactosides were in O-linked oligosaccharides. The strong staining with DSA was probably due to GlcNAc. Furthermore, sialylgalactosides with the sequence Neu5Ac(alpha2,3)Gal(beta1,4)GlcNAc in O-linked oligosaccharides have been shown by means of MAA, PNA and RCA-I.
Bendel-Stenzel,
The origin and migration of primordial germ cells in the mouse.
1998, Pubmed
Bendel-Stenzel,
The origin and migration of primordial germ cells in the mouse.
1998,
Pubmed
Bird,
Oligosaccharides containing fucose linked alpha(1-3) and alpha(1-4) to N-acetylglucosamine cause decompaction of mouse morulae.
1984,
Pubmed
Crowley,
Carbohydrate binding studies on the lectin from Datura stramonium seeds.
1984,
Pubmed
Debray,
Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins.
1981,
Pubmed
Delbos,
The role of the glycoconjugates in the migration of anuran amphibian germ cells.
1984,
Pubmed
,
Xenbase
Delbos,
[Detection, using fluorescent lectins, of specific carbohydrate chains in primordial germ cells of anuran amphibians].
1982,
Pubmed
,
Xenbase
Delbos,
[Inhibitory effect of lectins extracted from Arachis hypogea and soybeans on germ cell migration in the embryo of Xenopus laevis (Amphibia, Anura)].
1983,
Pubmed
,
Xenbase
Didier,
Expression and distribution of carbohydrate sequences in chick germ cells: a comparative study with lectins and the NC-1/HNK-1 monoclonal antibody.
1990,
Pubmed
Didier,
Different immunoreactivities of anti-soluble lactose lectin antisera to tissues from early chick embryos: a histochemical study.
1993,
Pubmed
Drickamer,
Biology of animal lectins.
1993,
Pubmed
Edelman,
Cell adhesion molecules: implications for a molecular histology.
1991,
Pubmed
Fazel,
Presence of a unique glycoconjugate on the surface of rat primordial germ cells during migration.
1987,
Pubmed
Fazel,
Glycoconjugate unique to migrating primordial germ cells differs with genera.
1990,
Pubmed
García-Castro,
Interactions between germ cells and extracellular matrix glycoproteins during migration and gonad assembly in the mouse embryo.
1997,
Pubmed
Gomperts,
Primordial germ cell migration.
1994,
Pubmed
,
Xenbase
Green,
Lectin affinity high-performance liquid chromatography. Interactions of N-glycanase-released oligosaccharides with Ricinus communis agglutinin I and Ricinus communis agglutinin II.
1987,
Pubmed
Heasman,
Contact relations and guidance of primordial germ cells on their migratory route in embryos of Xenopus laevis.
1981,
Pubmed
,
Xenbase
Hennigar,
Histochemical specificity of beta-galactose binding lectins from Arachis hypogaea (peanut) and Ricinus communis (castor bean).
1987,
Pubmed
Knibbs,
Characterization of the carbohydrate binding specificity of the leukoagglutinating lectin from Maackia amurensis. Comparison with other sialic acid-specific lectins.
1991,
Pubmed
Lasky,
Selectin-carbohydrate interactions and the initiation of the inflammatory response.
1995,
Pubmed
Lucocq,
Detection of terminal N-linked N-acetylglucosamine residues in the Golgi apparatus using galactosyltransferase and endoglucosaminidase F/peptide N-glycosidase F: adaptation of a biochemical approach to electron microscopy.
1987,
Pubmed
Madrid,
Lectin cytochemical characterization of the N- and O-linked oligosaccharides in the human rectum.
2000,
Pubmed
Madrid,
Subcellular characterization of glycoproteins in the principal cells of human gallbladder. A lectin cytochemical study.
1994,
Pubmed
Muramatsu,
Developmentally regulated expression of cell surface carbohydrates during mouse embryogenesis.
1988,
Pubmed
Ono,
Histochemical application of mild alkaline hydrolysis for selective elimination of O-glycosidically linked glycoproteins.
1983,
Pubmed
Sato,
Oncodevelopmental carbohydrate antigens: distribution of ECMA 2 and 3 antigens in embryonic and adult tissues of the mouse and in teratocarcinomas.
1986,
Pubmed
Schauer,
Chemistry, metabolism, and biological functions of sialic acids.
1982,
Pubmed
Shirane,
Role of peanut-lectin-affinity molecules (PLAM) on primordial germ cells in the initial determination of sex in anura.
1987,
Pubmed
Slack,
Regional biosynthetic markers in the early amphibian embryo.
1984,
Pubmed
Spicer,
Diversity of cell glycoconjugates shown histochemically: a perspective.
1992,
Pubmed
Stoddart,
Lectin histochemistry and cytochemistry-light microscopy : avidin-biotin amplification on resin-embedded sections.
1998,
Pubmed
Sáez,
Carbohydrate moieties of the interstitial and glandular tissues of the amphibian Pleurodeles waltl testis shown by lectin histochemistry.
2001,
Pubmed
Sáez,
Glycan residues of N- and O-linked oligosaccharides in the premeiotic spermatogenetic cells of the urodele amphibian Pleurodeles waltl characterize by means of lectin histochemistry.
2000,
Pubmed
Sáez,
Lectin histochemical localization of N- and O-linked oligosaccharides during the spermiogenesis of the urodele amphibian Pleurodeles waltl.
1999,
Pubmed
Turner,
Cell adhesion molecules: a unifying approach to topographic biology.
1992,
Pubmed
Urven,
Analysis of germ line development in the chick embryo using an anti-mouse EC cell antibody.
1988,
Pubmed
Yoshinaga,
Selective lectin-binding sites of primordial germ cells in chick and quail embryos.
1992,
Pubmed
Zalik,
Expression of the galactose-binding lectins during the formation of organ primordia in the chick embryo.
1994,
Pubmed