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Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing.
Borovjagin AV
,
Gerbi SA
.
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Mutations in the 5' portion of Xenopus U3 snoRNA were tested for function in oocytes. The results revealed a new cleavage site (A0) in the 3' region of vertebrate external transcribed spacer sequences. In addition, U3 mutagenesis uncoupled cleavage at sites 1 and 2, flanking the 5' and 3' ends of 18S rRNA, and generated novel intermediates: 19S and 18.5S pre-rRNAs. Furthermore, specific nucleotides in Xenopus U3 snoRNA that are required for cleavages in pre-rRNA were identified: box A is essential for site A0 cleavage, the GAC-box A' region is necessary for site 1 cleavage, and the 3' end of box A' and flanking nucleotides are required for site 2 cleavage. Differences between metazoan and yeast U3 snoRNA-mediated rRNA processing are enumerated. The data support a model where metazoan U3 snoRNA acts as a bridge to draw together the 5' and 3' ends of the 18S rRNA coding region within pre-rRNA to coordinate their cleavage.
Ajuh,
Xenopus borealis and Xenopus laevis 28S ribosomal DNA and the complete 40S ribosomal precursor RNA coding units of both species.
1991, Pubmed,
Xenbase
Ajuh,
Xenopus borealis and Xenopus laevis 28S ribosomal DNA and the complete 40S ribosomal precursor RNA coding units of both species.
1991,
Pubmed
,
Xenbase
Allmang,
Processing of the yeast pre-rRNA at sites A(2) and A(3) is linked.
1996,
Pubmed
Beltrame,
Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.
1992,
Pubmed
,
Xenbase
Beltrame,
Base pairing between U3 and the pre-ribosomal RNA is required for 18S rRNA synthesis.
1995,
Pubmed
Billy,
Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis.
2000,
Pubmed
Borovjagin,
U3 small nucleolar RNA is essential for cleavage at sites 1, 2 and 3 in pre-rRNA and determines which rRNA processing pathway is taken in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Borovjagin,
The spacing between functional Cis-elements of U3 snoRNA is critical for rRNA processing.
2000,
Pubmed
,
Xenbase
Chu,
The RNA of RNase MRP is required for normal processing of ribosomal RNA.
1994,
Pubmed
Colley,
Dhr1p, a putative DEAH-box RNA helicase, is associated with the box C+D snoRNP U3.
2000,
Pubmed
Dennis,
Formation of the 5' end pseudoknot in small subunit ribosomal RNA: involvement of U3-like sequences.
1997,
Pubmed
Elela,
RNase III cleaves eukaryotic preribosomal RNA at a U3 snoRNP-dependent site.
1996,
Pubmed
Enright,
5'ETS rRNA processing facilitated by four small RNAs: U14, E3, U17, and U3.
1996,
Pubmed
Eppens,
The roles of Rrp5p in the synthesis of yeast 18S and 5.8S rRNA can be functionally and physically separated.
1999,
Pubmed
Ganot,
Nucleolar factors direct the 2'-O-ribose methylation and pseudouridylation of U6 spliceosomal RNA.
1999,
Pubmed
Gerbi,
Small nucleolar RNA.
1995,
Pubmed
Hannon,
Accurate processing of human pre-rRNA in vitro.
1989,
Pubmed
Hartshorne,
Two 5'-ETS regions implicated in interactions with U3 snoRNA are required for small subunit rRNA maturation in Trypanosoma brucei.
1999,
Pubmed
Henry,
The 5' end of yeast 5.8S rRNA is generated by exonucleases from an upstream cleavage site.
1994,
Pubmed
Hughes,
Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
1991,
Pubmed
Hughes,
Functional base-pairing interaction between highly conserved elements of U3 small nucleolar RNA and the small ribosomal subunit RNA.
1996,
Pubmed
Intine,
Essential structural features in the Schizosaccharomyces pombe pre-rRNA 5' external transcribed spacer.
1999,
Pubmed
Jeppesen,
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA.
1988,
Pubmed
,
Xenbase
Kass,
Primary processing of mammalian rRNA involves two adjacent cleavages and is not species specific.
1987,
Pubmed
Kass,
The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
1990,
Pubmed
Kufel,
Yeast Rnt1p is required for cleavage of the pre-ribosomal RNA in the 3' ETS but not the 5' ETS.
1999,
Pubmed
Labhart,
Characterization of three sites of RNA 3' end formation in the Xenopus ribosomal gene spacer.
1986,
Pubmed
,
Xenbase
Lange,
Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.
1998,
Pubmed
,
Xenbase
Lange,
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
1998,
Pubmed
,
Xenbase
Lee,
Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10.
1997,
Pubmed
Lee,
Imp3p and Imp4p, two specific components of the U3 small nucleolar ribonucleoprotein that are essential for pre-18S rRNA processing.
1999,
Pubmed
Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed
Liang,
U14 base-pairs with 18S rRNA: a novel snoRNA interaction required for rRNA processing.
1995,
Pubmed
Lygerou,
Accurate processing of a eukaryotic precursor ribosomal RNA by ribonuclease MRP in vitro.
1996,
Pubmed
Méreau,
An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA.
1997,
Pubmed
Mishra,
Three small nucleolar RNAs that are involved in ribosomal RNA precursor processing.
1997,
Pubmed
,
Xenbase
Morrissey,
Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
1993,
Pubmed
Morrissey,
Birth of the snoRNPs: the evolution of RNase MRP and the eukaryotic pre-rRNA-processing system.
1995,
Pubmed
Mougey,
A U3 small nuclear ribonucleoprotein-requiring processing event in the 5' external transcribed spacer of Xenopus precursor rRNA.
1993,
Pubmed
,
Xenbase
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Peculis,
Disruption of U8 nucleolar snRNA inhibits 5.8S and 28S rRNA processing in the Xenopus oocyte.
1993,
Pubmed
,
Xenbase
Reddy,
Small nuclear RNAs and RNA processing.
1983,
Pubmed
Samarsky,
Functional mapping of the U3 small nucleolar RNA from the yeast Saccharomyces cerevisiae.
1998,
Pubmed
Savino,
Preribosomal RNA processing in Xenopus oocytes does not include cleavage within the external transcribed spacer as an early step.
1991,
Pubmed
,
Xenbase
Savino,
In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
1990,
Pubmed
,
Xenbase
Schmitt,
Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae.
1993,
Pubmed
Sharma,
Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.
1999,
Pubmed
Sharma,
The 5' end of the 18S rRNA can be positioned from within the mature rRNA.
1999,
Pubmed
Tollervey,
A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
1987,
Pubmed
Tycowski,
Requirement for intron-encoded U22 small nucleolar RNA in 18S ribosomal RNA maturation.
1994,
Pubmed
,
Xenbase
Udem,
The cytoplasmic maturation of a ribosomal precursor ribonucleic acid in yeast.
1973,
Pubmed
Venema,
Two distinct recognition signals define the site of endonucleolytic cleavage at the 5'-end of yeast 18S rRNA.
1995,
Pubmed
Venema,
Ribosome synthesis in Saccharomyces cerevisiae.
1999,
Pubmed
Venema,
RRP5 is required for formation of both 18S and 5.8S rRNA in yeast.
1996,
Pubmed
Young,
Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.
1978,
Pubmed
Yu,
Sequence requirements for maturation of the 5' terminus of human 18 S rRNA in vitro.
1992,
Pubmed
