Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Transforming growth factor beta (TGFbeta) signaling is transduced via Smad2-Smad4-DNA-binding protein complexes which bind to responsive elements in the promoters of target genes. However, the mechanism of how the complexes activate the target genes is unclear. Here we identify Xenopus Swift, a novel nuclear BRCT (BRCA1 C-terminal) domain protein that physically interacts with Smad2 via its BRCT domains. We examine the activity of Swift in relation to gene activation in Xenopus embryos. Swift mRNA has an expression pattern similar to that of Smad2. Swift has intrinsic transactivation activity and activates target gene transcription in a TGFbeta-Smad2-dependent manner. Inhibition of Swift activity results in the suppression of TGFbeta-induced gene transcription and defective mesendoderm development. Blocking Swift function affects neither bone morphogenic protein nor fibroblast growth factor signaling during early development. We conclude that Swift is a novel coactivator of Smad2 and that Swift has a critical role in embryonic TGFbeta-induced gene transcription. Our results suggest that Swift may be a general component of TGFbeta signaling.
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991, Pubmed,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Armes,
The ALK-2 and ALK-4 activin receptors transduce distinct mesoderm-inducing signals during early Xenopus development but do not co-operate to establish thresholds.
1997,
Pubmed
,
Xenbase
Callebaut,
From BRCA1 to RAP1: a widespread BRCT module closely associated with DNA repair.
1997,
Pubmed
Chen,
Smad4 and FAST-1 in the assembly of activin-responsive factor.
1997,
Pubmed
,
Xenbase
Chen,
A transcriptional partner for MAD proteins in TGF-beta signalling.
1996,
Pubmed
,
Xenbase
Conlon,
Inhibition of Xbra transcription activation causes defects in mesodermal patterning and reveals autoregulation of Xbra in dorsal mesoderm.
1996,
Pubmed
,
Xenbase
Ecochard,
A novel Xenopus mix-like gene milk involved in the control of the endomesodermal fates.
1998,
Pubmed
,
Xenbase
Escher,
Conservation of glutamine-rich transactivation function between yeast and humans.
2000,
Pubmed
Faure,
Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development.
2000,
Pubmed
,
Xenbase
Feng,
The tumor suppressor Smad4/DPC4 and transcriptional adaptor CBP/p300 are coactivators for smad3 in TGF-beta-induced transcriptional activation.
1998,
Pubmed
Frisch,
XBMPRII, a novel Xenopus type II receptor mediating BMP signaling in embryonic tissues.
1998,
Pubmed
,
Xenbase
Fuks,
DNA methyltransferase Dnmt1 associates with histone deacetylase activity.
2000,
Pubmed
Germain,
Homeodomain and winged-helix transcription factors recruit activated Smads to distinct promoter elements via a common Smad interaction motif.
2000,
Pubmed
,
Xenbase
Graff,
Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily.
1996,
Pubmed
,
Xenbase
Green,
Graded changes in dose of a Xenopus activin A homologue elicit stepwise transitions in embryonic cell fate.
1990,
Pubmed
,
Xenbase
Green,
Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm.
1992,
Pubmed
,
Xenbase
Gurdon,
Activin signalling and response to a morphogen gradient.
1994,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Henry,
Mixer, a homeobox gene required for endoderm development.
1998,
Pubmed
,
Xenbase
Hoodless,
Dominant-negative Smad2 mutants inhibit activin/Vg1 signaling and disrupt axis formation in Xenopus.
1999,
Pubmed
,
Xenbase
Janknecht,
TGF-beta-stimulated cooperation of smad proteins with the coactivators CBP/p300.
1998,
Pubmed
Jaynes,
Active repression of transcription by the engrailed homeodomain protein.
1991,
Pubmed
Kato,
Neuralization of the Xenopus embryo by inhibition of p300/ CREB-binding protein function.
1999,
Pubmed
,
Xenbase
Koonin,
BRCA1 protein products ... Functional motifs..
1996,
Pubmed
Labbé,
Smad2 and Smad3 positively and negatively regulate TGF beta-dependent transcription through the forkhead DNA-binding protein FAST2.
1998,
Pubmed
,
Xenbase
LaBonne,
Mesoderm induction by activin requires FGF-mediated intracellular signals.
1994,
Pubmed
,
Xenbase
Lechner,
PTIP, a novel BRCT domain-containing protein interacts with Pax2 and is associated with active chromatin.
2000,
Pubmed
Margolis,
cDNAs with long CAG trinucleotide repeats from human brain.
1997,
Pubmed
Massagué,
Transcriptional control by the TGF-beta/Smad signaling system.
2000,
Pubmed
Massagué,
TGF-beta signal transduction.
1998,
Pubmed
McDowell,
Activin has direct long-range signalling activity and can form a concentration gradient by diffusion.
1997,
Pubmed
,
Xenbase
Pouponnot,
Physical and functional interaction of SMADs and p300/CBP.
1998,
Pubmed
Ryan,
Eomesodermin, a key early gene in Xenopus mesoderm differentiation.
1996,
Pubmed
,
Xenbase
Sadowski,
GAL4-VP16 is an unusually potent transcriptional activator.
1988,
Pubmed
Shiama,
The p300/CBP family: integrating signals with transcription factors and chromatin.
1997,
Pubmed
Shimizu,
Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade.
1998,
Pubmed
,
Xenbase
Shimizu,
A quantitative analysis of signal transduction from activin receptor to nucleus and its relevance to morphogen gradient interpretation.
1999,
Pubmed
,
Xenbase
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Takaishi,
Regulation of cell-cell adhesion by rac and rho small G proteins in MDCK cells.
1997,
Pubmed
Vojtek,
Mammalian Ras interacts directly with the serine/threonine kinase Raf.
1993,
Pubmed
Watanabe,
FAST-1 is a key maternal effector of mesoderm inducers in the early Xenopus embryo.
1999,
Pubmed
,
Xenbase
Weinstein,
Neural induction in Xenopus laevis: evidence for the default model.
1997,
Pubmed
,
Xenbase
Wilson,
Concentration-dependent patterning of the Xenopus ectoderm by BMP4 and its signal transducer Smad1.
1997,
Pubmed
,
Xenbase
Zhou,
Characterization of human FAST-1, a TGF beta and activin signal transducer.
1998,
Pubmed
,
Xenbase
Zhu,
Smad3 mutant mice develop metastatic colorectal cancer.
1998,
Pubmed
,
Xenbase
Zorn,
Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3 physically interact with beta-catenin.
1999,
Pubmed
,
Xenbase
