XB-ART-9667
J Virol
2001 Mar 01;755:2314-23. doi: 10.1128/JVI.75.5.2314-2323.2001.
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Human GLI-2 is a tat activation response element-independent Tat cofactor.
Browning CM
,
Smith MJ
,
Clark NM
,
Lane BR
,
Parada C
,
Montano M
,
KewalRamani VN
,
Littman DR
,
Essex M
,
Roeder RG
,
Markovitz DM
.
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Zinc finger-containing GLI proteins are involved in the development of Caenorhabditis elegans, Xenopus, Drosophila, zebrafish, mice, and humans. In this study, we show that an isoform of human GLI-2 strongly synergizes with the Tat transactivating proteins of human immunodeficiency virus types 1 and 2 (HIV-1 and -2) and markedly stimulates viral replication. GLI-2 also synergizes with the previously described Tat cofactor cyclin T1 to stimulate Tat function. Surprisingly, GLI-2/Tat synergy is not dependent on either a typical GLI DNA binding site or an intact Tat activation response element but does require an intact TATA box. Thus, GLI-2/Tat synergy results from a mechanism of action which is novel both for a GLI protein and for a Tat cofactor. These findings link the GLI family of transcriptional and developmental regulatory proteins to Tat function and HIV replication.
???displayArticle.pubmedLink??? 11160734
???displayArticle.pmcLink??? PMC114814
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???displayArticle.grants??? [+]
AI30924 NIAID NIH HHS , AI36685 NIAID NIH HHS , K08-AI01293 NIAID NIH HHS , T32 GM008353 NIGMS NIH HHS , T32 GM145304 NIGMS NIH HHS , T32 GM007863 NIGMS NIH HHS , T32 CA009676 NCI NIH HHS
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