XB-ART-9701
Biochem Biophys Res Commun
2000 Apr 13;2702:581-7. doi: 10.1006/bbrc.2000.2454.
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Modulation of the Ca(2+)-activated Cl(-) channel by 14-3-3epsilon.
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We have previously reported an association of 14-3-3epsilon isoform with calmodulin. Using the voltage-clamp technique, the present study investigated the potential role of 14-3-3 in modulating the Ca(2+)-activated Cl(-) channel (CaCC) endogenously expressed in Xenopus oocytes. Injection of 14-3-3epsilon antisense oligodeoxynucleotides resulted in potentiation of the ionomycin-induced Cl(-) current, while 14-3-3 peptide and calmodulin inhibitor, W13, suppressed the antisense-potentiated current. The data suggest that 14-3-3epsilon plays an inhibitory role in modulating the CaCC by interacting with the calmodulin-dependent pathway. The potential role of 14-3-3epsilon in other tissues and its therapeutic potential for cystic fibrosis are discussed.
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Species referenced: Xenopus
Genes referenced: clca1.3 ywhae