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XB-ART-9726
FEBS Lett 2000 Dec 29;4872:277-81. doi: 10.1016/s0014-5793(00)02370-x.
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Activation of Xenopus Chk1 by mutagenesis of threonine-377.

Wang SX , Dunphy WG .


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Xenopus Chk1 (Xchk1) is required for the checkpoint-associated delay of the cell cycle in frog egg extracts containing unreplicated or UV-damaged DNA. Phosphorylation of Xchk1 at multiple sites in the SQ/TQ domain (residues 314-366) in response to unreplicated or UV-damaged DNA results in elevation of its kinase activity. We have found that mutagenesis of Thr-377 in the conserved Thr-Arg-Phe (TRF) motif of Xchk1 also leads to a substantial increase in kinase activity. Thr-377 does not appear to be a site of phosphorylation in Xchk1. These findings suggest that Thr-377 may play a role in suppressing the activity of Xchk1.

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Species referenced: Xenopus
Genes referenced: chek1