Kuriyama S et al. (2000), Xerl: a novel secretory protein expressed in ey...

XB-ART-11117

Mech Dev 2000 May 01;931-2:233-7. doi: 10.1016/s0925-4773(00)00280-x.

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Xerl: a novel secretory protein expressed in eye and brain of Xenopus embryo.

Kuriyama S , Miyatani S , Kinoshita T .

Abstract
A novel gene, Xerl (Xenopus EGF-like repeat with laminin-G domain protein) was isolated from a Xenopus head cDNA library prepared from tailbud. This gene encoded 779 amino acids including a potential signal sequence, twelve EGF-like repeats, a laminin-G domain, a RGD sequence and a VWF motif. In the EGF-like repeat and the laminin-G domain, Xerl showed similarity to those of Drosophila Crumbs, respectively. Zygotic expression of Xerl began at late gastrula, and increased through neurula up to the tailbud stage. In adult organs, Xerl was detected in brain and eye. Whole-mount in situ hybridization showed that Xerl expression occurred first in the anterior bilateral region of neurula and gradually localized to retina and forebrain and boundaries of midbrain and hindbrain.

PubMed ID: 10781962
Article link: Mech Dev

Species referenced: Xenopus
Genes referenced: crb2 egf nr2e1 pax6 vwf

Article Images: [+] show captions

	Fig. 4. Whole-mount in situ hybridization of Xerl. (A) Dorsal view (st.15). Anterior is left. Arrowhead indicates posterior part of neural groove. (B) Anterior view of (A). Arrows show anterior part of neural plate. (C) Dorsal view (st.17). Anterior is left. Arrowhead indicates anteriorly bilateral expression. (D) Anterior view of (C). Arrowheads indicate anteriorly bilateral expression. Arrow indicates hypothalamus primodia. (E) Dorsal view (st.20). Anterior is left. Arrowhead indicates neural tube. (F) Anterior view of (E). Arrowheads indicate eye anlage and future midbrain. (G) Dorsal view (st.24). Anterior is left. (H) Anterior view of (G). Arrowheads indicate same region in (D,F). (I) Dorsal view (st.28). (J) Anterior view of (I). Arrowhead indicates midbrain expression. Arrow shows diencephalic expression. (K) Xpax6 expression (st.25). (L) Anterior view of (K). White arrowhead indicates blank of expression at midbrain. (M,M′) Xpax6 expression (st.32). (N,N′) Xtll expression (st.32). (O,O′) Xerl expression (st.32). (M–O) Lateral view. (M′–O′) Highly magnified dorsal view. (P,Q) Transverse section of the whole embryo stained with Xerl probe at st.32. (P) Midbrain. (Q) Lens vesicle. Arrows indicate remarkable expression at the lens equator zone. (R) In situ hybridization on the tissue sectioned at diencephalic level (st.32). Arrowheads indicate Xerl expression near the apical surface of ventricular zone. Arrow indicates Xerl expression of outer layer of neural retina. mb, midbrain; rm, rhombomere; mhb, mid-hindbrain boundary; te, telencephalon; di, diencephalon; ey, eye; gu, gut; lv, lens vesicle; nr, neural retina; dt, dorsal thalamus; ht, hypothalamus; ls, lens.
	Fig. 4. Crb2 expression at stg. 32
	Fig. 4. Nr2e1 expression at stg 32