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Int J Dev Biol 2005 Jan 01;498:939-51. doi: 10.1387/ijdb.052083nt.
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Systematic screening for genes specifically expressed in the anterior neuroectoderm during early Xenopus development.

Takahashi N , Tochimoto N , Ohmori SY , Mamada H , Itoh M , Inamori M , Shinga J , Osada S , Taira M .

A cDNA library derived from the anterior neuroectoderm (ANE) of Xenopus late-gastrula embryos was systematically screened to isolate novel developmental regulatory genes involved in early brain development. We isolated 1,706 5 expressed sequence tags (ESTs), which were subdivided into 1,383 clusters and categorized into 19 classes based on predicted functions according to their similarities to other known genes. Of these, 757 clusters that were considered possible novel regulatory genes or unknown genes were subjected to expression pattern analysis using whole-mount in situ hybridization. Genes from 69 clusters (9%) were expressed in the ANE region. Based on their expression patterns and predicted amino acid sequences, 25 genes were selected for further analysis as novel Xenopus genes expressed broadly or region-specifically in the ANE. Eighteen genes were expressed in postulated patterning centers in the neuroectoderm, including the anterior (four genes) and lateral (nine genes) neural ridges, the midbrain-hindbrain boundary region (one gene) and the midline region of the neural plate (two genes), whereas 13 genes were expressed in the eye anlagen. Therefore, early regionalization of the neuroectoderm appears to occur mainly in those neural patterning centers and the eye anlagen. We determined the entire coding regions of p54nrb, Semaphorin 6D and a novel gene designated scribble-related protein 1 (SCRP1). Interestingly, Semaphorin 6D is expressed in the mesoderm with a dorsoventral gradient, as well as in the ectoderm at the gastrula stage, implying a new role for this protein in development other than in axon guidance.

PubMed ID: 16281171
Article link: Int J Dev Biol

Species referenced: Xenopus laevis
Genes referenced: cdkn2aip cirbp cripto.3 crx eif4a3.1 epha2 etv1 fgfr4 foxa4 fzd7 gmnn hes4 hesx1 lemd3 lhx5 lrrc1 nono nppa nrp1 otx1 otx2 pax2 pdcd10 prickle1 psen1 ran rax rbpj rhoa rpl32 rrm1 rrm2.2 sema6d sfrp2 six3 sox11 sox2 sox3 tbx2 tcf3 utp15 XB972976 [provisional:rpl32] zic1 zic3

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