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Int J Dev Biol 2005 Jan 01;497:781-96. doi: 10.1387/ijdb.052003ep.
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Exploration of the extracellular space by a large-scale secretion screen in the early Xenopus embryo.

Pera EM , Hou S , Strate I , Wessely O , De Robertis EM .

Secreted proteins play a crucial role in intercellular communication during embryogenesis and in the adult. We recently described a novel method, designated as secretion cloning, that allows identifying extracellular proteins exclusively based on their ability to be secreted by transfected cells. In this paper, we present the results of a large-scale screening of more than 90,000 clones from three cDNA expression libraries constructed from early Xenopus embryos. Of 170 sequenced clones, 65 appeared to encode secreted proteins; 26 clones (40%) were identical to previously known Xenopus genes, 25 clones (38%) were homologous to other genes identified in various organisms and 14 clones (22%) were novel. Apart from these bona fide secreted proteins, we also isolated lysosomal or other secretory pathway proteins and some cytoplasmic proteins commonly found in body fluids. Among the novel secreted proteins were two putative growth factors of the Granulin family, termed xGra1 and xGra2; they are structurally similar to EGF and TGFalpha and show a spotted expression pattern in the epidermis. Another secreted protein, designated xSOUL, belongs to the family of heme-binding proteins and exhibits distinct expression in the early brain. A third protein, termed Xystatin, is related to cysteine proteinase inhibitors. Our results indicate that secretion cloning is an effective and generally useful tool for the unbiased isolation of secreted proteins.

PubMed ID: 16172975
Article link: Int J Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: acp5 actb actg1 admp adsl akr1b1 aldh1a2 aldoc cer1 chmp1b chmp2a cndp2 cst3 ctsb ctsd ctsl ctsv darmin dctpp1 dnaja2 dnajb11 egf enpp2 fbp1 frzb frzb2 fth1.1 gamt gdi2 glul gpc4 grn has1 hebp2 hexb hnrnpa1 hsbp1 htra1 igfbp5 ism1 kdelr2 ldhb lefty lfng lman1 lman2 man2b1 manf mdh1 nbl1 nodal3.1 nog nucb1 pabpc1 pdia3 pepd pgam1 pkm plbd2 plg ppib ppp4c psap rdh10 rhoa rpe serpine2 serpinh1 sfrp2 shmt1 smarcc1 spint2 stmn1 szl taldo1 tinf2 tmem170a tor1a tpm3 tpt1 ubqln4 urod vcp ykt6 ywhab ywhae ywhaq ywhaz zp4

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