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J Cell Biol 1999 Jan 25;1442:225-40. doi: 10.1083/jcb.144.2.225.
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Temporal differences in the appearance of NEP-B78 and an LBR-like protein during Xenopus nuclear envelope reassembly reflect the ordered recruitment of functionally discrete vesicle types.

Drummond S , Ferrigno P , Lyon C , Murphy J , Goldberg M , Allen T , Smythe C , Hutchison CJ .

In this work, we have used novel mAbs against two proteins of the endoplasmic reticulum and outer nuclear membrane, termed NEP-B78 and p65, in addition to a polyclonal antibody against the inner nuclear membrane protein LBR (lamin B receptor), to study the order and dynamics of NE reassembly in the Xenopus cell-free system. Using these reagents, we demonstrate differences in the timing of recruitment of their cognate membrane proteins to the surface of decondensing chromatin in both the cell-free system and XLK-2 cells. We show unequivocally that, in the cell-free system, two functionally and biochemically distinct vesicle types are necessary for NE assembly. We find that the process of distinct vesicle recruitment to chromatin is an ordered one and that NEP-B78 defines a vesicle population involved in the earliest events of reassembly in this system. Finally, we present evidence that NEP-B78 may be required for the targeting of these vesicles to the surface of decondensing chromatin in this system. The results have important implications for the understanding of the mechanisms of nuclear envelope disassembly and reassembly during mitosis and for the development of systems to identify novel molecules that control these processes.

PubMed ID: 9922450
PMC ID: PMC2132889
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: krt62 lamtor3 lbr lss nucb1 rela runx2

Article Images: [+] show captions
References [+] :
Acharya, The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events. 1995, Pubmed