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Mol Cell Biol
1999 Feb 01;192:1427-37. doi: 10.1128/MCB.19.2.1427.
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Regulation of ribosomal S6 protein kinase-p90(rsk), glycogen synthase kinase 3, and beta-catenin in early Xenopus development.
Torres MA
,
Eldar-Finkelman H
,
Krebs EG
,
Moon RT
.
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beta-Catenin is a multifunctional protein that binds cadherins at the plasma membrane, HMG box transcription factors in the nucleus, and several cytoplasmic proteins that are involved in regulating its stability. In developing embryos and in some human cancers, the accumulation of beta-catenin in the cytoplasm and subsequently the nuclei of cells may be regulated by the Wnt-1 signaling cascade and by glycogen synthase kinase 3 (GSK-3). This has increased interest in regulators of both GSK-3 and beta-catenin. Searching for kinase activities able to phosphorylate the conserved, inhibitory-regulatory GSK-3 residue serine 9, we found p90(rsk) to be a potential upstream regulator of GSK-3. Overexpression of p90(rsk) in Xenopus embryos leads to increased steady-state levels of total beta-catenin but not of the free soluble protein. Instead, p90(rsk) overexpression increases the levels of beta-catenin in a cell fraction containing membrane-associated cadherins. Consistent with the lack of elevation of free beta-catenin levels, ectopic p90(rsk) was unable to rescue dorsal cell fate in embryos ventralized by UV irradiation. We show that p90(rsk) is a downstream target of fibroblast growth factor (FGF) signaling during early Xenopus development, since ectopic FGF signaling activates both endogenous and overexpressed p90(rsk). Moreover, overexpression of a dominant negative FGF receptor, which blocks endogenous FGF signaling, leads to decreased p90(rsk) kinase activity. Finally, we report that FGF inhibits endogenous GSK-3 activity in Xenopus embryos. We hypothesize that FGF and p90(rsk) play heretofore unsuspected roles in modulating GSK-3 and beta-catenin.
Aberle,
beta-catenin is a target for the ubiquitin-proteasome pathway.
1997, Pubmed
Aberle,
beta-catenin is a target for the ubiquitin-proteasome pathway.
1997,
Pubmed
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Amaya,
FGF signalling in the early specification of mesoderm in Xenopus.
1993,
Pubmed
,
Xenbase
Bjørbaek,
Divergent functional roles for p90rsk kinase domains.
1995,
Pubmed
Bradham,
Transrepression of type II collagen by TGF-beta and FGF is protein kinase C dependent and is mediated through regulatory sequences in the promoter and first intron.
1994,
Pubmed
Cadigan,
Wnt signaling: a common theme in animal development.
1997,
Pubmed
,
Xenbase
Christian,
Xwnt-8 modifies the character of mesoderm induced by bFGF in isolated Xenopus ectoderm.
1992,
Pubmed
,
Xenbase
Cook,
Wingless inactivates glycogen synthase kinase-3 via an intracellular signalling pathway which involves a protein kinase C.
1996,
Pubmed
Cornell,
FGF is a prospective competence factor for early activin-type signals in Xenopus mesoderm induction.
1995,
Pubmed
,
Xenbase
Cornell,
Activin-mediated mesoderm induction requires FGF.
1994,
Pubmed
,
Xenbase
Cross,
Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B.
,
Pubmed
,
Xenbase
Dominguez,
Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos.
1995,
Pubmed
,
Xenbase
Du,
Identification of distinct classes and functional domains of Wnts through expression of wild-type and chimeric proteins in Xenopus embryos.
1995,
Pubmed
,
Xenbase
Eldar-Finkelman,
Inactivation of glycogen synthase kinase-3 by epidermal growth factor is mediated by mitogen-activated protein kinase/p90 ribosomal protein S6 kinase signaling pathway in NIH/3T3 cells.
1995,
Pubmed
Eldar-Finkelman,
Expression and characterization of glycogen synthase kinase-3 mutants and their effect on glycogen synthase activity in intact cells.
1996,
Pubmed
Erikson,
Purification and characterization of a protein kinase from Xenopus eggs highly specific for ribosomal protein S6.
1986,
Pubmed
,
Xenbase
Erikson,
Antibodies to Xenopus egg S6 kinase II recognize S6 kinase from progesterone- and insulin-stimulated Xenopus oocytes and from proliferating chicken embryo fibroblasts.
1987,
Pubmed
,
Xenbase
Erikson,
A protein kinase from Xenopus eggs specific for ribosomal protein S6.
1985,
Pubmed
,
Xenbase
Fagotto,
Binding to cadherins antagonizes the signaling activity of beta-catenin during axis formation in Xenopus.
1996,
Pubmed
,
Xenbase
Fisher,
Evidence for two catalytically active kinase domains in pp90rsk.
1996,
Pubmed
Funayama,
Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.
1995,
Pubmed
,
Xenbase
Giebelhaus,
Changes in the expression of alpha-fodrin during embryonic development of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Goode,
Differential regulation of glycogen synthase kinase-3 beta by protein kinase C isotypes.
1992,
Pubmed
Gotoh,
Microtubule-associated-protein (MAP) kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogen-activated MAP kinase of fibroblastic cells.
1990,
Pubmed
Gotoh,
Involvement of the MAP kinase cascade in Xenopus mesoderm induction.
1995,
Pubmed
,
Xenbase
Gumbiner,
Signal transduction of beta-catenin.
1995,
Pubmed
,
Xenbase
Gumbiner,
Carcinogenesis: a balance between beta-catenin and APC.
1997,
Pubmed
Hartley,
MAP kinase is activated during mesoderm induction in Xenopus laevis.
1994,
Pubmed
,
Xenbase
Hayashi,
A Drosophila homolog of the tumor suppressor gene adenomatous polyposis coli down-regulates beta-catenin but its zygotic expression is not essential for the regulation of Armadillo.
1997,
Pubmed
He,
Glycogen synthase kinase-3 and dorsoventral patterning in Xenopus embryos.
1995,
Pubmed
,
Xenbase
Hinck,
Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing beta-catenin binding to the cell adhesion protein cadherin.
1994,
Pubmed
Hoppler,
Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos.
1996,
Pubmed
,
Xenbase
Ikeda,
Axin, a negative regulator of the Wnt signaling pathway, forms a complex with GSK-3beta and beta-catenin and promotes GSK-3beta-dependent phosphorylation of beta-catenin.
1998,
Pubmed
Isaacs,
eFGF regulates Xbra expression during Xenopus gastrulation.
1994,
Pubmed
,
Xenbase
Jones,
A Xenopus ribosomal protein S6 kinase has two apparent kinase domains that are each similar to distinct protein kinases.
1988,
Pubmed
,
Xenbase
Kameshita,
A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel.
1989,
Pubmed
Kemler,
From cadherins to catenins: cytoplasmic protein interactions and regulation of cell adhesion.
1993,
Pubmed
Kimelman,
Synergistic principles of development: overlapping patterning systems in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Klein,
A molecular mechanism for the effect of lithium on development.
1996,
Pubmed
,
Xenbase
LaBonne,
Mesoderm induction by activin requires FGF-mediated intracellular signals.
1994,
Pubmed
,
Xenbase
Larabell,
Establishment of the dorso-ventral axis in Xenopus embryos is presaged by early asymmetries in beta-catenin that are modulated by the Wnt signaling pathway.
1997,
Pubmed
,
Xenbase
Levine,
Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
1994,
Pubmed
,
Xenbase
McGrew,
Wnt and FGF pathways cooperatively pattern anteroposterior neural ectoderm in Xenopus.
1997,
Pubmed
,
Xenbase
Miller,
Signal transduction through beta-catenin and specification of cell fate during embryogenesis.
1996,
Pubmed
Miller,
Analysis of the signaling activities of localization mutants of beta-catenin during axis specification in Xenopus.
1997,
Pubmed
,
Xenbase
Morin,
Activation of beta-catenin-Tcf signaling in colon cancer by mutations in beta-catenin or APC.
1997,
Pubmed
Moule,
Regulation of protein kinase B and glycogen synthase kinase-3 by insulin and beta-adrenergic agonists in rat epididymal fat cells. Activation of protein kinase B by wortmannin-sensitive and -insensitive mechanisms.
1997,
Pubmed
Munemitsu,
Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein.
1995,
Pubmed
Orford,
Serine phosphorylation-regulated ubiquitination and degradation of beta-catenin.
1997,
Pubmed
Papkoff,
Regulation of complexed and free catenin pools by distinct mechanisms. Differential effects of Wnt-1 and v-Src.
1997,
Pubmed
Pierce,
Regulation of Spemann organizer formation by the intracellular kinase Xgsk-3.
1995,
Pubmed
,
Xenbase
Polakis,
Mutations in the APC gene and their implications for protein structure and function.
1995,
Pubmed
Rocheleau,
Wnt signaling and an APC-related gene specify endoderm in early C. elegans embryos.
1997,
Pubmed
Rubinfeld,
Binding of GSK3beta to the APC-beta-catenin complex and regulation of complex assembly.
1996,
Pubmed
Rubinfeld,
Association of the APC gene product with beta-catenin.
1993,
Pubmed
Rubinfeld,
Stabilization of beta-catenin by genetic defects in melanoma cell lines.
1997,
Pubmed
Saito,
The mechanism by which epidermal growth factor inhibits glycogen synthase kinase 3 in A431 cells.
1994,
Pubmed
Sakanaka,
Bridging of beta-catenin and glycogen synthase kinase-3beta by axin and inhibition of beta-catenin-mediated transcription.
1998,
Pubmed
Schönwasser,
Activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway by conventional, novel, and atypical protein kinase C isotypes.
1998,
Pubmed
Stambolic,
Lithium inhibits glycogen synthase kinase-3 activity and mimics wingless signalling in intact cells.
1996,
Pubmed
,
Xenbase
Stambolic,
Mitogen inactivation of glycogen synthase kinase-3 beta in intact cells via serine 9 phosphorylation.
1994,
Pubmed
Stefanovic,
Activation of a ribosomal protein S6 protein kinase in Xenopus oocytes by insulin and insulin-receptor kinase.
1986,
Pubmed
,
Xenbase
Sturgill,
Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.
1988,
Pubmed
,
Xenbase
Su,
Association of the APC tumor suppressor protein with catenins.
1993,
Pubmed
Sutherland,
Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling.
1993,
Pubmed
Torres,
Activities of the Wnt-1 class of secreted signaling factors are antagonized by the Wnt-5A class and by a dominant negative cadherin in early Xenopus development.
1996,
Pubmed
,
Xenbase
Tsuda,
Induction of protein kinase C activation and Ca2+ mobilization by fibroblast growth factor in Swiss 3T3 cells.
1985,
Pubmed
Umbhauer,
Mesoderm induction in Xenopus caused by activation of MAP kinase.
1995,
Pubmed
,
Xenbase
Vleminckx,
Adenomatous polyposis coli tumor suppressor protein has signaling activity in Xenopus laevis embryos resulting in the induction of an ectopic dorsoanterior axis.
1997,
Pubmed
,
Xenbase
Woodgett,
Regulation and functions of the glycogen synthase kinase-3 subfamily.
1994,
Pubmed
Yang-Snyder,
A frizzled homolog functions in a vertebrate Wnt signaling pathway.
1996,
Pubmed
,
Xenbase
Yost,
The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3.
1996,
Pubmed
,
Xenbase
van Leeuwen,
Biological activity of soluble wingless protein in cultured Drosophila imaginal disc cells.
1994,
Pubmed
