Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1998 Dec 15;1724:7430-41. doi: 10.1093/emboj/17.24.7430.
Show Gene links
Show Anatomy links
The role of exportin-t in selective nuclear export of mature tRNAs.
Arts GJ
,
Kuersten S
,
Romby P
,
Ehresmann B
,
Mattaj IW
.
???displayArticle.abstract???
Exportin-t (Xpo-t) is a vertebrate nuclear export receptor for tRNAs that binds tRNA cooperatively with GTP-loaded Ran. Xpo-t antibodies are shown to efficiently block tRNA export from Xenopus oocyte nuclei suggesting that it is responsible for at least the majority of tRNA export in these cells. We examine the mechanism by which Xpo-t-RanGTP specifically exports mature tRNAs rather than other forms of nuclear RNA, including tRNA precursors. Chemical and enzymatic footprinting together with phosphate modification interference reveals an extensive interaction between the backbone of the TPsiC and acceptor arms of tRNAPhe and Xpo-t-RanGTP. Analysis of mutant or precursor tRNA forms demonstrates that, aside from these recognition elements, accurate 5' and 3' end-processing of tRNA affects Xpo-t-RanGTP interaction and nuclear export, while aminoacylation is not essential. Intron-containing, end-processed, pre-tRNAs can be bound by Xpo-t-RanGTP and are rapidly exported from the nucleus if Xpo-t is present in excess. These results suggest that at least two mechanisms are involved in discrimination of pre-tRNAs and mature tRNAs prior to nuclear export.
Altman,
Recent studies of ribonuclease P.
1993, Pubmed
Altman,
Recent studies of ribonuclease P.
1993,
Pubmed
Arts,
Identification of a nuclear export receptor for tRNA.
1998,
Pubmed
,
Xenbase
Behlen,
Lead-catalyzed cleavage of yeast tRNAPhe mutants.
1990,
Pubmed
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Brown,
Crystallographic and biochemical investigation of the lead(II)-catalyzed hydrolysis of yeast phenylalanine tRNA.
1985,
Pubmed
Brown,
Pb(II)-catalysed cleavage of the sugar-phosphate backbone of yeast tRNAPhe--implications for lead toxicity and self-splicing RNA.
,
Pubmed
Corbett,
Nucleocytoplasmic transport of macromolecules.
1997,
Pubmed
Dahlberg,
Functions of the GTPase Ran in RNA export from the nucleus.
1998,
Pubmed
De Robertis,
Intranuclear location of the tRNA splicing enzymes.
1981,
Pubmed
,
Xenbase
Di Nicola Negri,
The eucaryal tRNA splicing endonuclease recognizes a tripartite set of RNA elements.
1997,
Pubmed
Edqvist,
Pleiotrophic effects of point mutations in yeast tRNA(Asp) on the base modification pattern.
1993,
Pubmed
,
Xenbase
Fabbri,
Conservation of substrate recognition mechanisms by tRNA splicing endonucleases.
1998,
Pubmed
,
Xenbase
Fornerod,
The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88.
1997,
Pubmed
Fornerod,
CRM1 is an export receptor for leucine-rich nuclear export signals.
1997,
Pubmed
,
Xenbase
Giegé,
tRNA structure and aminoacylation efficiency.
1993,
Pubmed
Grüter,
TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus.
1998,
Pubmed
,
Xenbase
Görlich,
Nuclear protein import.
1997,
Pubmed
Görlich,
A novel class of RanGTP binding proteins.
1997,
Pubmed
,
Xenbase
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase
Hellmuth,
Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA.
1998,
Pubmed
Hopper,
Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.
1980,
Pubmed
Hurt,
Cloning and characterization of LOS1, a Saccharomyces cerevisiae gene that affects tRNA splicing.
1987,
Pubmed
Hüttenhofer,
Hydroxyl radical cleavage of tRNA in the ribosomal P site.
1992,
Pubmed
Izaurralde,
RNA export.
1995,
Pubmed
Izaurralde,
A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export.
1997,
Pubmed
,
Xenbase
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Izaurralde,
Transport of macromolecules between the nucleus and the cytoplasm.
1998,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Kim,
Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.
1974,
Pubmed
Kutay,
Identification of a tRNA-specific nuclear export receptor.
1998,
Pubmed
,
Xenbase
Kutay,
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.
1997,
Pubmed
,
Xenbase
Ladner,
Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
1975,
Pubmed
Latham,
Defining the inside and outside of a catalytic RNA molecule.
1989,
Pubmed
Lee,
A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export.
1996,
Pubmed
Lentzen,
Structure of 4.5S RNA in the signal recognition particle of Escherichia coli as studied by enzymatic and chemical probing.
1996,
Pubmed
Li,
RNA minihelices as model substrates for ATP/CTP:tRNA nucleotidyltransferase.
1997,
Pubmed
Lin-Marq,
A yeast RNA binding protein that resembles the human autoantigen La.
1995,
Pubmed
Liu,
Recognition of the universally conserved 3'-CCA end of tRNA by elongation factor EF-Tu.
1998,
Pubmed
Lockard,
Mapping tRNA structure in solution using double-strand-specific ribonuclease V1 from cobra venom.
1981,
Pubmed
Massire,
DRAWNA: a program for drawing schematic views of nucleic acids.
1994,
Pubmed
Mattaj,
Nucleocytoplasmic transport: the soluble phase.
1998,
Pubmed
McClain,
Model substrates for an RNA enzyme.
1987,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Nazarenko,
Many of the conserved nucleotides of tRNA(Phe) are not essential for ternary complex formation and peptide elongation.
1994,
Pubmed
Negrutskii,
Eukaryotic translation elongation factor 1 alpha: structure, expression, functions, and possible role in aminoacyl-tRNA channeling.
1998,
Pubmed
Nishikura,
RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in the spliced transfer RNA.
1981,
Pubmed
,
Xenbase
Nissen,
Crystal structure of the ternary complex of Phe-tRNAPhe, EF-Tu, and a GTP analog.
1995,
Pubmed
Ohno,
Nucleocytoplasmic transport: the last 200 nanometers.
1998,
Pubmed
Piñol-Roma,
hnRNP proteins: localization and transport between the nucleus and the cytoplasm.
1993,
Pubmed
Rether,
Studies on tRNA nucleotidyltransferase from baker's yeast. 1. Purification of the enzyme. Protection against thermal inactivation and inhibition by several substrates.
1974,
Pubmed
Rio,
Accurate and efficient pre-mRNA splicing in Drosophila cell-free extracts.
1988,
Pubmed
Romby,
Comparison of the tertiary structure of yeast tRNA(Asp) and tRNA(Phe) in solution. Chemical modification study of the bases.
1987,
Pubmed
Romby,
Yeast tRNAAsp tertiary structure in solution and areas of interaction of the tRNA with aspartyl-tRNA synthetase. A comparative study of the yeast phenylalanine system by phosphate alkylation experiments with ethylnitrosourea.
1985,
Pubmed
Rudinger,
Minimalist aminoacylated RNAs as efficient substrates for elongation factor Tu.
1994,
Pubmed
Sampson,
Recognition of yeast tRNA(Phe) by its cognate yeast phenylalanyl-tRNA synthetase: an analysis of specificity.
1992,
Pubmed
Sampson,
Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.
1989,
Pubmed
Schimmel,
An operational RNA code for amino acids and possible relationship to genetic code.
1993,
Pubmed
Segref,
Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores.
1997,
Pubmed
Shi,
CCA addition by tRNA nucleotidyltransferase: polymerization without translocation?
1998,
Pubmed
Simos,
The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases.
1996,
Pubmed
Simos,
Nuclear pore proteins are involved in the biogenesis of functional tRNA.
1996,
Pubmed
Stade,
Exportin 1 (Crm1p) is an essential nuclear export factor.
1997,
Pubmed
Varshney,
Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase.
1991,
Pubmed
Vlassov,
Tertiary structure of tRNAs in solution monitored by phosphodiester modification with ethylnitrosourea.
1981,
Pubmed
Werner,
Specific cleavages of pure tRNAs by plumbous ions.
1976,
Pubmed
