Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Subtle determinants of the nucleocytoplasmic partitioning of in vivo-transcribed RNase MRP RNA in Xenopus laevis oocytes.
Jeong-Yu S
,
Davis AF
,
Clayton DA
.
???displayArticle.abstract???
RNase MRP is a ribonucleoprotein originally identified on the basis of its ability to cleave RNA endonucleolytically from origins of mitochondrial DNA replication, rendering it a likely candidate for a role in priming leading-strand synthesis of mtDNA. In addition, a nuclear role for RNase MRP has been identified in yeast (Saccharomyces cerevisiae) ribosomal RNA processing. Consistent with a duality of function, RNase MRP has been localized to both mitochondria and nucleoli by in situ techniques. The RNA component of this ribonucleoprotein has been characterized from several different species. We previously cloned the gene for Xenopus laevis MRP RNA and showed that RNase MRP RNA is differentially expressed during amphibian development; in addition, the microinjected X. laevis RNase MRP RNA gene is correctly and efficiently transcribed in vivo. This article presents an analysis of the intracellular movement of in vivo-transcribed RNase MRP RNA in microinjected mature X. laevis oocytes. Although X. laevis MRP RNA is assembled into a ribonucleoprotein form and transported in an expected manner, human and mouse MRP RNAs exhibit markedly different transport patterns even though they are highly conserved in primary sequence. Furthermore, the only currently assigned protein (Th autoantigen) binding site in MRP RNA can be deleted without loss of nuclear export capacity. These results indicate that subtle determinants must exist for nucleocytoplasmic partitioning of this RNP and that the conserved Th autoantigen binding region appears unnecessary for the transit of in vivo-transcribed MRP RNA to the cytoplasm of mature X. laevis oocytes.
Bataillé,
Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
1990, Pubmed,
Xenbase
Bataillé,
Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process.
1990,
Pubmed
,
Xenbase
Bennett,
Characterization of a Xenopus laevis ribonucleoprotein endoribonuclease. Isolation of the RNA component and its expression during development.
1992,
Pubmed
,
Xenbase
Borer,
Major nucleolar proteins shuttle between nucleus and cytoplasm.
1989,
Pubmed
,
Xenbase
Chang,
A novel endoribonuclease cleaves at a priming site of mouse mitochondrial DNA replication.
1987,
Pubmed
Chang,
A mammalian mitochondrial RNA processing activity contains nucleus-encoded RNA.
1987,
Pubmed
Chang,
Mouse RNAase MRP RNA is encoded by a nuclear gene and contains a decamer sequence complementary to a conserved region of mitochondrial RNA substrate.
1989,
Pubmed
Chu,
The RNA of RNase MRP is required for normal processing of ribosomal RNA.
1994,
Pubmed
Clayton,
A nuclear function for RNase MRP.
1994,
Pubmed
Dargemont,
Export of mRNA from microinjected nuclei of Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Davis,
Distribution of RNase MRP RNA during Xenopus laevis oogenesis.
1995,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Dworetzky,
Translocation of RNA-coated gold particles through the nuclear pores of oocytes.
1988,
Pubmed
,
Xenbase
Gold,
The RNA processing enzyme RNase MRP is identical to the Th RNP and related to RNase P.
1989,
Pubmed
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Karwan,
Nuclear RNase MRP processes RNA at multiple discrete sites: interaction with an upstream G box is required for subsequent downstream cleavages.
1991,
Pubmed
Kiss,
Plant small nuclear RNAs. Nucleolar U3 snRNA is present in plants: partial characterization.
1985,
Pubmed
Kiss,
Evidence against a mitochondrial location of the 7-2/MRP RNA in mammalian cells.
1992,
Pubmed
Li,
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
1990,
Pubmed
Li,
Subcellular partitioning of MRP RNA assessed by ultrastructural and biochemical analysis.
1994,
Pubmed
Lygerou,
The POP1 gene encodes a protein component common to the RNase MRP and RNase P ribonucleoproteins.
1994,
Pubmed
Maquat,
Nuclear mRNA export.
1991,
Pubmed
Matera,
A perinucleolar compartment contains several RNA polymerase III transcripts as well as the polypyrimidine tract-binding protein, hnRNP I.
1995,
Pubmed
Mattaj,
Small nuclear RNAs in messenger RNA and ribosomal RNA processing.
1993,
Pubmed
Nigg,
Nuclear import-export: in search of signals and mechanisms.
1991,
Pubmed
Paluh,
Schizosaccharomyces pombe RNase MRP RNA is homologous to metazoan RNase MRP RNAs and may provide clues to interrelationships between RNase MRP and RNase P.
1995,
Pubmed
,
Xenbase
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Riegel,
A novel transcriptional activator (PO-B) binds between the TATA box and cap site of the pro-opiomelanocortin gene.
1990,
Pubmed
Schmitt,
Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae.
1993,
Pubmed
Schmitt,
Secondary structure of RNase MRP RNA as predicted by phylogenetic comparison.
1993,
Pubmed
Schmitt,
Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viability.
1992,
Pubmed
Schmitt,
Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain.
1994,
Pubmed
Simons,
Analysis of the intracellular localization and assembly of Ro ribonucleoprotein particles by microinjection into Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Terns,
Retention and 5' cap trimethylation of U3 snRNA in the nucleus.
1994,
Pubmed
,
Xenbase
Topper,
Secondary structure of the RNA component of a nuclear/mitochondrial ribonucleoprotein.
1990,
Pubmed
Topper,
Characterization of human MRP/Th RNA and its nuclear gene: full length MRP/Th RNA is an active endoribonuclease when assembled as an RNP.
1990,
Pubmed
Topper,
A role for RNAase MRP in mitochondrial RNA processing.
1992,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Yuan,
5' flanking sequences of human MRP/7-2 RNA gene are required and sufficient for the transcription by RNA polymerase III.
1991,
Pubmed
Yuan,
The 40-kilodalton to autoantigen associates with nucleotides 21 to 64 of human mitochondrial RNA processing/7-2 RNA in vitro.
1991,
Pubmed
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
