Zon LI , Mather C , Burgess S , Bolce ME , Harland RM , Orkin SH .

GM42341 NIGMS NIH HHS , HL02347 NHLBI NIH HHS , HL32259 NHLBI NIH HHS

	FIG. 1. Comparison of the predicted amino acid sequences of vertebrate GATA-binding proteins. The zinc-finger DNA-binding domain is underlined. The conserved amino acids outside the DNA-binding domain are marked (*). x, Xenopus; c, chicken; mn, mouse; h, human. (For sequences, see refs. 14, 15, 18-23, and 46.)
	FIG. 2. Distinctive features of the finger region of the vertebrate GATA-binding proteins. Amino acids 314-409 of the finger domains of mGATA-1 are shown at the top with positions of divergence noted by X. Amino acid residues characteristic of the various GATA family members are shown below. Intervening sequence position is marked based on the structure of the murine GATA-1 gene (30).
	FIG. 3. Expression of GATA-binding protein genes in Xenopus erythroid cells. Total cell RNAs (15 ,ug) were subjected to Northern blot analysis and hybridized with a xGATA-1B cDNA probe (A) or a xGATA-3 probe (B). Cells studied include Xenopus eggs or oocytes (egg), tadpole erythroid cells, adult erythroid cells, adult reticulocytes (retic), XTC cell line, XL cell line, murine erythroleukemia cells (MEL), and human erythroleukemia cells (K562).
	FIG. 4. Developmental expression of xGATA-binding protein and larval globin genes. (A) Total cellular RNAs (1 /g) from staged Xenopus embryos were subjected to RT-PCR using specific primers for ,T1 globin, xGATA-1, xGATA-2, xGATA-3, and xMyoD. Controls consisted of RT-PCR products from total RNA from Xenopus adult erythroid cells and HeLa cells and H20. Lanes: 1, oocyte; 2, 4-cell embryo; 3, 32-cell embryo; 13, erythroid cells; 14, HeLa cells; 15, H20. The Nieuwkoop stage is indicated in the following lanes. Lanes: 4, 7; 5, 9; 6, 11; 7, 12.5; 8, 13; 9, 18; 10, 24; 11, 26; 12, 30. (B) Total cellular RNAs from staged Xenopus embryos were subjected to Northern blot analysis and hybridized with an aT1 globin cDNA probe (37). Nieuwkoop stages are as follows. Lanes: 1, 10.5; 2, 12; 3, 13; 4, 15; 5, 17; 6, 20; 7, 24; 8, 26; 9, 30; 10, 33; 11, 38; 12, erythroid-cell control.

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