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Mol Cell Biol 1991 Dec 01;1112:5985-91. doi: 10.1128/mcb.11.12.5985-5991.1991.
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tpr-met oncogene product induces maturation-producing factor activation in Xenopus oocytes.

Daar IO , White GA , Schuh SM , Ferris DK , Vande Woude GF .

tpr-met, a tyrosine kinase oncogene, is the activated form of the met proto-oncogene that encodes the receptor for hepatocyte growth factor/scatter factor. The tpr-met product (p65tpr-met) was tested for its ability to induce meiotic maturation in Xenopus oocytes. While src and abl tyrosine kinase oncogene products have previously been shown to be inactive in this assay, p65tpr-met efficiently induced maturation-promoting factor (MPF) activation and germinal vesicle breakdown (GVBD) together with the associated increase in ribosomal S6 subunit phosphorylation. tpr-met-mediated MPF activation and GVBD was dependent on the endogenous c-mosxe, while the increase in S6 protein phosphorylation was not significantly affected by the loss of mos function. The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine inhibits tpr-met-mediated GVBD at concentrations that prevent insulin- but not progesterone-induced oocyte maturation. Moreover, maturation triggered by tpr-met is also inhibited by cyclic AMP-dependent protein kinase. This is the first demonstration that a tyrosine kinase oncogene product, p65tpr-met, can induce meiotic maturation in Xenopus oocytes and activate MPF through a mos-dependent pathway, possibly the insulin or insulinlike growth factor 1 pathway.

PubMed ID: 1719375
PMC ID: PMC361762
Article link: Mol Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: abl1 cdk1 ins mos tpr

References [+] :
Allende, Comparison of in vivo and in vitro properties of cyclic adenosine 3':5'-monophosphate phosphodiesterase of amphibian oocytes. 1977, Pubmed, Xenbase