Xenbase is undergoing scheduled maintenance Wednesday, June 14 and Thursday, June 15, 2023. Xenbase will be unavailable on those days.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Proc Natl Acad Sci U S A 1990 Feb 01;874:1521-5.
Show Gene links Show Anatomy links

A serum factor that activates the phosphatidylinositol phosphate signaling system in Xenopus oocytes.

Tigyi G , Dyer D , Matute C , Miledi R .

Blood sera from many vertebrate species elicit large oscillatory chloride currents in oocytes from the frog Xenopus laevis. Rabbit serum was active at dilutions as great as one part in 10 million. Intracellularly applied serum was ineffective, and externally applied serum failed to trigger oscillatory currents when the intracellular level of ionized calcium was prevented from rising by loading the oocyte with EGTA. The serum also caused an increase of inositol 1,4,5-trisphosphate in the oocyte. We conclude that serum contains a factor which activates a membrane receptor that is coupled to the phosphatidylinositol second messenger system. The active factor is a protein with an apparent molecular mass of 60-70 kDa in gel permeation chromatography. Although the normal function of the serum factor is still unknown, it may have far-reaching implications, because it acts on the multifunctional phosphatidylinositol phosphate signaling system. Also, because of its great potency the serum factor and Xenopus oocytes are very useful for probing the operation of the phosphatidylinositol system.

PubMed ID: 1689488
PMC ID: PMC53507
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

References [+] :
Berridge, Inositol phosphates and cell signalling. 1989, Pubmed