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Cell Tissue Res 1986 Jan 01;2452:445-51.
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Determination of human factor VIII (AHG-associated protein)--antigen in endothelial cells from Xenopus laevis (XTH cells). Evaluation of a sensitive ELISA technique.

Schlage WK .

AHG-associated protein (AHG-a.p.), the antigen of the blood-clotting factor VIII complex, is a specific endothelial cell marker. Primary (p-XTH) and established (XTH-2) endothelial cells from the hearts of Xenopus laevis tadpoles were assayed for the presence of this marker by means of immunological cross-reaction (recognition of common antigenic sites) with antiserum against human AHG-a.p. Radial immunodiffusion and rocket immunoelectrophoresis proved to be insufficiently sensitive, whereas immunofluorescence and a newly evaluated ELISA technique gave positive results. The very high sensitivity of the ELISA (less than 1/240,000 of the AHG-a.p. in 0.1 ml human standard plasma can be detected) and the removal of interfering proteins by gel filtration also revealed the presence of AHG-a.p. in the fetal calf serum used in the culture medium; earlier investigations into this subject by a one-step radioimmunoassay had reported negative results. Specially adapted XTH-2 cells were grown in a protein-and serum-free hydrolysate medium in order to demonstrate the presence of a Xenopus-derived antigen that was immunoreactive with the anti-human AHG-a.p.

PubMed ID: 3091251

Species referenced: Xenopus laevis
Genes referenced: f8

References [+] :
Bereiter-Hahn, Interaction of metabolic inhibitors with actin fibrils. 1984, Pubmed, Xenbase