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XB-ART-29298
Dev Biol 1985 Mar 01;1081:210-9.
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Immunological relationship between oocyte nuclear proteins of Xenopus laevis and X. borealis.

Dreyer C , Wang YH , Hausen P .


Abstract
Monoclonal antibodies (mABs) have been raised against oocyte nuclear proteins of Xenopus laevis and X. borealis and have been screened for species specificity. Although about 40% of all germinal vesicle polypeptides differ between the two species as judged by two-dimensional gel analysis, most mABs react with polypeptides of both species. Biochemical analysis of the antigens by immunoblotting revealed that a homologue of each antigen of one species could be detected in the other species, despite frequent differences in molecular structure. Nevertheless, five strictly species-specific mABs have been identified. All five are directed against the same acidic polypeptide B3 of X. borealis, which is a structurally altered homologue of the protein N1, previously described in X. laevis germinal vesicles. In oocyte nuclei of hybrids between X. laevis females and X. borealis males, polypeptide of both species appear to be accumulated equivalently. Exceptions to this rule are most easily explained by differences between individuals and by the loss of certain alleles resulting from the cross.

PubMed ID: 3882485
Article link: Dev Biol


Species referenced: Xenopus laevis
Genes referenced: igf2bp3
Antibodies: Ahcy Ab1 Germinal Vesicle Ab1 Germinal Vesicle Ab2 Ncl Ab1 Npm1 Ab1 Transketolase Ab2 Xnf7 Ab2 transketolase Ab1