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Dev Biol 2008 Jul 01;3191:86-99. doi: 10.1016/j.ydbio.2008.04.007.
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PACSIN2 regulates cell adhesion during gastrulation in Xenopus laevis.

We previously identified the adaptor protein PACSIN2 as a negative regulator of ADAM13 proteolytic function. In Xenopus embryos, PACSIN2 is ubiquitously expressed, suggesting that PACSIN2 may control other proteins during development. To investigate this possibility, we studied PACSIN2 function during Xenopus gastrulation and in XTC cells. Our results show that PACSIN2 is localized to the plasma membrane via its coiled-coil domain. We also show that increased levels of PACSIN2 in embryos inhibit gastrulation, fibronectin (FN) fibrillogenesis and the ability of ectodermal cells to spread on a FN substrate. These effects require PACSIN2 coiled-coil domain and are not due to a reduction of FN or integrin expression and/or trafficking. The expression of a Mitochondria Anchored PACSIN2 (PACSIN2-MA) sequesters wild type PACSIN2 to mitochondria, and blocks gastrulation without interfering with cell spreading or FN fibrillogenesis but perturbs both epiboly and convergence/extension. In XTC cells, the over-expression of PACSIN2 but not PACSIN2-MA prevents the localization of integrin beta1 to focal adhesions (FA) and filamin to stress fiber. PACSIN2-MA prevents filamin localization to membrane ruffles but not to stress fiber. We propose that PACSIN2 may regulate gastrulation by controlling the population of activated alpha5beta1 integrin and cytoskeleton strength during cell movement.

PubMed ID: 18495106
PMC ID: PMC2494715
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: acta4 actl6a adam13 bcr chrd flna fn1 gal.2 itga5 itgav itgb1 myc pacsin2 tbx2 tbxt tubb2b
Antibodies: Adam13 Ab1 Flna Ab1 Fluro-phalloidin Ab Fn1 Ab1 Fn1 Ab3 Itgav Ab1 Itgb1 Ab1 Itgb1 Ab3 Mitochondria Lectin Myc Ab5 Pacsin2 Ab1
Morpholinos: pacsin2 MO1 pacsin2 MO2

Article Images: [+] show captions
References [+] :
Alfandari, Xenopus ADAM 13 is a metalloprotease required for cranial neural crest-cell migration. 2001, Pubmed, Xenbase