Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell Commun Adhes
2008 May 01;151:119-32. doi: 10.1080/15419060802013885.
Show Gene links
Show Anatomy links
Trafficking dynamics of glycosylated pannexin 1 proteins.
Boassa D
,
Qiu F
,
Dahl G
,
Sosinsky G
.
???displayArticle.abstract???
Pannexins are mammalian orthologs of innexins and have a predicted topological folding pattern similar to that of connexins, except they are glycosylated. Rat pannexin 1 is glycosylated at N254 and this residue is important for plasma membrane targeting. Here we demonstrate that cell surface expression levels of the rat pannexin 1 N254Q mutant are rescued by coexpression with the wild-type protein. In paired Xenopus oocytes, the functional effect of this rescue is inconsequential; however, cell surface deglycosylation by PNGase F significantly enhanced functional gap junction formation. In mammalian cells, wild-type oligomers traffic at a slower rate than Myc-or tetracysteine domain-tagged versions, a behavior opposite to that of tagged connexins. The temporal differences of Panx1 trafficking correlate with spatial differences of intracellular localizations induced by Golgi blockage by Brefeldin-A or glycosylation prevention by tunicamycin. Therefore, Panx1 has kinetics and dynamics that make it unique to serve distinct functions separate from connexin-based channels.
Bao,
Innexins form two types of channels.
2007, Pubmed,
Xenbase
Bao,
Innexins form two types of channels.
2007,
Pubmed
,
Xenbase
Bao,
Pannexin membrane channels are mechanosensitive conduits for ATP.
2004,
Pubmed
,
Xenbase
Boassa,
Pannexin1 channels contain a glycosylation site that targets the hexamer to the plasma membrane.
2007,
Pubmed
Bruzzone,
Pannexins, a family of gap junction proteins expressed in brain.
2003,
Pubmed
,
Xenbase
Bukauskas,
Clustering of connexin 43-enhanced green fluorescent protein gap junction channels and functional coupling in living cells.
2000,
Pubmed
Contreras,
Gating and regulation of connexin 43 (Cx43) hemichannels.
2003,
Pubmed
Dahl,
Pannexin: to gap or not to gap, is that a question?
2006,
Pubmed
Gaietta,
Multicolor and electron microscopic imaging of connexin trafficking.
2002,
Pubmed
Gerido,
Connexin disorders of the ear, skin, and lens.
2004,
Pubmed
Huang,
Pannexin1 is expressed by neurons and glia but does not form functional gap junctions.
2007,
Pubmed
Hunter,
Zonula occludens-1 alters connexin43 gap junction size and organization by influencing channel accretion.
2005,
Pubmed
Jordan,
Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells.
1999,
Pubmed
Khanna,
Glycosylation increases potassium channel stability and surface expression in mammalian cells.
2001,
Pubmed
,
Xenbase
Laird,
Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells.
1995,
Pubmed
Laird,
Life cycle of connexins in health and disease.
2006,
Pubmed
Levine,
Cell-cell channel formation and lectins.
1991,
Pubmed
,
Xenbase
Lippincott-Schwartz,
Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
1989,
Pubmed
Lippincott-Schwartz,
Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic.
1991,
Pubmed
Litvin,
What is hidden in the pannexin treasure trove: the sneak peek and the guesswork.
2006,
Pubmed
Locovei,
Pannexin 1 in erythrocytes: function without a gap.
2006,
Pubmed
,
Xenbase
Locovei,
Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex.
2007,
Pubmed
,
Xenbase
Martin,
Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity.
2005,
Pubmed
Musil,
Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER.
1993,
Pubmed
,
Xenbase
Musil,
Regulation of connexin degradation as a mechanism to increase gap junction assembly and function.
2000,
Pubmed
Panchin,
Evolution of gap junction proteins--the pannexin alternative.
2005,
Pubmed
Pelegrin,
Pannexin-1 mediates large pore formation and interleukin-1beta release by the ATP-gated P2X7 receptor.
2006,
Pubmed
Pelegrin,
Pannexin-1 couples to maitotoxin- and nigericin-induced interleukin-1beta release through a dye uptake-independent pathway.
2007,
Pubmed
Penuela,
Pannexin 1 and pannexin 3 are glycoproteins that exhibit many distinct characteristics from the connexin family of gap junction proteins.
2007,
Pubmed
Petrecca,
N-linked glycosylation sites determine HERG channel surface membrane expression.
1999,
Pubmed
Sosinsky,
The C-terminus of connexin43 adopts different conformations in the Golgi and gap junction as detected with structure-specific antibodies.
2007,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Vanden Abeele,
Functional implications of calcium permeability of the channel formed by pannexin 1.
2006,
Pubmed
Wang,
Inhibition of glycosylation induces formation of open connexin-43 cell-to-cell channels and phosphorylation and triton X-100 insolubility of connexin-43.
1995,
Pubmed
Watanabe,
Glycosylation affects the protein stability and cell surface expression of Kv1.4 but Not Kv1.1 potassium channels. A pore region determinant dictates the effect of glycosylation on trafficking.
2004,
Pubmed
