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Dev Biol 2009 Dec 01;3361:68-75. doi: 10.1016/j.ydbio.2009.09.033.
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The involvement of lethal giant larvae and Wnt signaling in bottle cell formation in Xenopus embryos.

Choi SC , Sokol SY .

Lethal giant larvae (Lgl) plays a critical role in establishment of cell polarity in epithelial cells. While Frizzled/Dsh signaling has been implicated in the regulation of the localization and activity of Lgl, it remains unclear whether specific Wnt ligands are involved. Here we show that Wnt5a triggers the release of Lgl from the cell cortex into the cytoplasm with the concomitant decrease in Lgl stability. The observed changes in Lgl localization were independent of atypical PKC (aPKC), which is known to influence Lgl distribution. In ectodermal cells, both Wnt5a and Lgl triggered morphological and molecular changes characteristic of apical constriction, whereas depletion of their functions prevented endogenous and ectopic bottle cell formation. Furthermore, Lgl RNA partially rescued bottle cell formation in embryos injected with a dominant negative Wnt5a construct. These results suggest a molecular link between Wnt5a and Lgl that is essential for apical constriction during vertebrate gastrulation.

PubMed ID: 19782678
PMC ID: PMC2801549
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a dvl1 dvl2 inhbb llgl1 mlc1 prkci wnt5a
Morpholinos: llgl1 MO1 llgl1 MO2

Article Images: [+] show captions
References [+] :
Barros, Drosophila nonmuscle myosin II promotes the asymmetric segregation of cell fate determinants by cortical exclusion rather than active transport. 2003, Pubmed