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Dev Dyn 2009 Jun 01;2386:1346-57. doi: 10.1002/dvdy.21920.
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CDK9/cyclin complexes modulate endoderm induction by direct interaction with Mix.3/mixer.

Zhu H , Doherty JR , Kuliyev E , Mead PE .

Mix-related homeodomain proteins are involved in endoderm formation in the early vertebrate embryo. We used a yeast two-hybrid screen to identify proteins that interact with Mix.3/mixer to regulate endoderm induction. We demonstrate that cyclin-dependent kinase 9 (CDK9) interacts with the carboxyl terminal domain of Mix.3. CDK9 is the catalytic subunit of the PTEF-b transcription elongation complex that phosphorylates the C-terminal domain of RNA polymerase II to promote efficient elongation of nascent transcripts. Using whole embryo transcription reporter and animal pole explant assays, we show that Mix.3 activity is regulated by CDK9/cyclin complexes. Co-expression of cyclin T2 and cyclin K had different effects on Mix.3 transcriptional activity and endoderm induction. Our data suggest that binding of CDK9, and the recruitment of different cyclin partners, can modulate the endoderm-inducing activity of Mix.3 during embryonic development. Developmental Dynamics 238:1346-1357, 2009. (c) 2009 Wiley-Liss, Inc.

PubMed ID: 19347956
PMC ID: PMC2832930
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: a2m ccnk ccnt2 cdk9 darmin gal.2 mixer sox17a sox17b.1 tbxt tbxt.2

Article Images: [+] show captions
References [+] :
Agius, Endodermal Nodal-related signals and mesoderm induction in Xenopus. 2000, Pubmed, Xenbase