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PLoS One 2010 Apr 08;54:e10158. doi: 10.1371/journal.pone.0010158.
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Manipulating heat shock factor-1 in Xenopus tadpoles: neuronal tissues are refractory to exogenous expression.

Dirks RP , van Geel R , Hensen SM , van Genesen ST , Lubsen NH .

The aging related decline of heat shock factor-1 (HSF1) signaling may be causally related to protein aggregation diseases. To model such disease, we tried to cripple HSF1 signaling in the Xenopus tadpole.Over-expression of heat shock factor binding protein-1 did not inhibit the heat shock response in Xenopus. RNAi against HSF1 mRNA inhibited the heat shock response by 70% in Xenopus A6 cells, but failed in transgenic tadpoles. Expression of XHSF380, a dominant-negative HSF1 mutant, was embryonic lethal, which could be circumvented by delaying expression via a tetracycline inducible promoter. HSF1 signaling is thus essential for embryonic Xenopus development. Surprisingly, transgenic expression of the XHSF380 or of full length HSF1, whether driven by a ubiquitous or a neural specific promoter, was not detectable in the larval brain.Our finding that the majority of neurons, which have little endogenous HSF1, refused to accept transgene-driven expression of HSF1 or its mutant suggests that HSF1 levels are strictly controlled in neuronal tissue.

PubMed ID: 20405018
PMC ID: PMC2854154
Article link: PLoS One

Species referenced: Xenopus
Genes referenced: hsbp1 hsf1 hsp70 hsp90aa1.1 hspa1l myc

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References [+] :
Balch, Adapting proteostasis for disease intervention. 2008, Pubmed