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Mol Cell Neurosci 2010 Sep 01;451:47-58. doi: 10.1016/j.mcn.2010.05.008.
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In vivo evidence for the involvement of the carboxy terminal domain in assembling connexin 36 at the electrical synapse.

Helbig I , Sammler E , Eliava M , Bolshakov AP , Rozov A , Bruzzone R , Monyer H , Hormuzdi SG .

Connexin 36 (Cx36)-containing electrical synapses contribute to the timing and amplitude of neural responses in many brain regions. A Cx36-EGFP transgenic was previously generated to facilitate their identification and study. In this study we demonstrate that electrical coupling is normal in transgenic mice expressing Cx36 from the genomic locus and suggest that fluorescent puncta present in brain tissue represent distributed electrical synapses. These qualities emphasize the usefulness of the Cx36-EGFP reporter as a tool for the detailed anatomical characterization of electrical synapses in fixed and living tissue. However, though the fusion protein is able to form gap junctions between Xenopus laevis oocytes it is unable to restore electrical coupling to interneurons in the Cx36-deficient mouse. Further experiments in transgenic tissue and non-neural cell lines reveal impaired transport to the plasma membrane as the possible cause. By analyzing the functional deficits exhibited by the fusion protein in vivo and in vitro, we identify a motif within Cx36 that may interact with other trafficking or scaffold proteins and thereby be responsible for its incorporation into electrical synapses.

PubMed ID: 20510366
PMC ID: PMC3025355
Article link: Mol Cell Neurosci
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: abcb6 calb1 calb2 gjd2 kit ocm3 ocm4.5 sp5 tbx2

Article Images: [+] show captions
References [+] :
Arumugam, NMDA receptors regulate developmental gap junction uncoupling via CREB signaling. 2005, Pubmed