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Proc Natl Acad Sci U S A 2010 Nov 09;10745:19344-9. doi: 10.1073/pnas.1008954107.
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Xenopus furry contributes to release of microRNA gene silencing.

Goto T , Fukui A , Shibuya H , Keller R , Asashima M .

A transcriptional corepressor, Xenopus furry (Xfurry), is expressed in the chordamesodermal region and induces secondary dorsal axes when overexpressed on the ventral side of the embryo. The N-terminal furry domain functions as a repressor, and the C-terminal leucine zipper (LZ) motifs /coiled-coil structure, found only in vertebrate homologs, contributes to the nuclear localization. The engrailed repressor (enR)+LZ repressor construct, which has properties similar to Xfurry, induced several chordamesodermal genes. In contrast, an antisense morpholino oligonucleotide, Xfurry-MO, and the activating construct, herpes simplex virus protein (VP16)+LZ, had effects opposite those of Xfurry overexpression. Because blocking protein synthesis with cycloheximide superinduced several Xfurry transcriptional targets, and because expression of enR+LZ induced such genes under cycloheximide treatment, we analyzed the role of an Xfurry transcriptional target, microRNA miR-15. Cycloheximide reduced the expression of primary miR-15 (pri-miR-15), whereas miR-15 reduced the expression of genes superinduced by cycloheximide treatment. These results show that Xfurry regulates chordamesodermal genes by contributing to repression of pretranscriptional gene silencing by miR-15.

PubMed ID: 20974966
PMC ID: PMC2984173
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cer1 chrd foxa4 fry gsc nodal5.2 not odc1 stk38 stk38l vegt
Morpholinos: foxa4 MO1 fry MO1 gsc MO1

Article Images: [+] show captions
References [+] :
Chiba, MST2- and Furry-mediated activation of NDR1 kinase is critical for precise alignment of mitotic chromosomes. 2009, Pubmed