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Nat Commun 2011 Jan 01;2:310. doi: 10.1038/ncomms1301.
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An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation.

Tena JJ , Alonso ME , de la Calle-Mustienes E , Splinter E , de Laat W , Manzanares M , Gómez-Skarmeta JL .

Developmental gene clusters are paradigms for the study of gene regulation; however, the mechanisms that mediate phenomena such as coregulation and enhancer sharing remain largely elusive. Here we address this issue by analysing the vertebrate Irx clusters. We first present a deep enhancer screen of a 2-Mbp span covering the IrxA cluster. Using chromosome conformation capture, we show that enhancer sharing is widespread within the cluster, explaining its evolutionarily conserved organization. We also identify a three-dimensional architecture, probably formed through interactions with CCCTC-binding factor, which is present within both Irx clusters of mouse, Xenopus and zebrafish. This architecture brings the promoters of the first two genes together in the same chromatin landscape. We propose that this unique and evolutionarily conserved genomic architecture of the vertebrate Irx clusters is essential for the coregulation of the first two genes and simultaneously maintains the third gene in a partially independent regulatory landscape.

PubMed ID: 21556064
Article link: Nat Commun

Species referenced: Xenopus
Genes referenced: irx1 irx2 irx4 mbp

Article Images: [+] show captions
References [+] :
Alarcón, A dual requirement for Iroquois genes during Xenopus kidney development. 2008, Pubmed, Xenbase