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Dev Dyn 2011 Jul 01;2407:1727-36. doi: 10.1002/dvdy.22671.
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The functions of maternal Dishevelled 2 and 3 in the early Xenopus embryo.

Tadjuidje E , Cha SW , Louza M , Wylie C , Heasman J .

Of the three Dishevelled (Dvl) genes, only Dvl2 and Dvl3 are maternally encoded in the frog, Xenopus laevis. We show here by loss of function analysis that single depletion of either Dvl2 or Dvl3 from the oocyte causes the same embryonic phenotype. We find that the effects of loss of function of Dvl2 and 3 together are additive, and that the proteins physically interact, suggesting that both are required in the same complex. We show that maternal Dvl2 and 3 are required for convergence extension movements downstream of the dorsally localized signaling pathway activated by Xnr3, but not downstream of the pathway activated by activin. Also, depletion of maternal Dvl2 and 3 mRNAs causes the up-regulation of a subset of zygotic ectodermal genes, including Foxi1e, with surprisingly no significant effect on the canonical Wnt direct target genes Siamois and Xnr3. We suggest that the likely reason for continued expression of the Wnt target genes in Dvl2/3-depleted embryos is that maternal Dvl mRNA depletion is insufficient to deplete stored punctae of Dvl protein in the oocyte cortex, which may transduce dorsal signaling after fertilization.

PubMed ID: 21618643
Article link: Dev Dyn
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: acod1lb dvl1 dvl2 dvl3 fgf3 foxi1 foxi2 hes5.10 isyna1 klf6 mapk8 msgn1 myc myod1 nodal3.1 nodal3.2 odc1 pcdh8 sia1 tbxt vegt
Antibodies: Dvl2 Ab1
Morpholinos: dvl2 MO2

Article Images: [+] show captions