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Abstract
RNAi has revolutionized reverse genetics; however, RNAi is not necessarily ubiquitous or constitutive. Lund and colleagues (pp. 1121-1131) show that microRNA (miRNA) effector Argonautes (Agos) are limiting and easily saturated during early Xenopus embryogenesis. Moreover, this stage is devoid of slicing capacity. Supplementation of Ago proteins rescued endogenous miRNA activity in the presence of exogenous siRNAs, and, excitingly, ectopic Ago2 could now support RNAi in Xenopus. These observations may potentially facilitate RNAi in other recalcitrant model organisms.
Armisen,
Abundant and dynamically expressed miRNAs, piRNAs, and other small RNAs in the vertebrate Xenopus tropicalis.
2009, Pubmed,
Xenbase
Armisen,
Abundant and dynamically expressed miRNAs, piRNAs, and other small RNAs in the vertebrate Xenopus tropicalis.
2009,
Pubmed
,
Xenbase
Cheloufi,
A dicer-independent miRNA biogenesis pathway that requires Ago catalysis.
2010,
Pubmed
Chendrimada,
TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing.
2005,
Pubmed
Cifuentes,
A novel miRNA processing pathway independent of Dicer requires Argonaute2 catalytic activity.
2010,
Pubmed
Clemens,
PKR--a protein kinase regulated by double-stranded RNA.
1997,
Pubmed
Diederichs,
Coexpression of Argonaute-2 enhances RNA interference toward perfect match binding sites.
2008,
Pubmed
Diederichs,
Dual role for argonautes in microRNA processing and posttranscriptional regulation of microRNA expression.
2007,
Pubmed
Elbashir,
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells.
2001,
Pubmed
Fellmann,
Functional identification of optimized RNAi triggers using a massively parallel sensor assay.
2011,
Pubmed
Fire,
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.
1998,
Pubmed
Fjose,
Inhibition of the microRNA pathway in zebrafish by siRNA.
2010,
Pubmed
Giraldez,
Zebrafish MiR-430 promotes deadenylation and clearance of maternal mRNAs.
2006,
Pubmed
Gregory,
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing.
2005,
Pubmed
Grimm,
Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver.
2010,
Pubmed
Grimm,
Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways.
2006,
Pubmed
Grishok,
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.
2001,
Pubmed
Hulstrand,
The use of antisense oligonucleotides in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Jackson,
Widespread siRNA "off-target" transcript silencing mediated by seed region sequence complementarity.
2006,
Pubmed
Khan,
Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs.
2009,
Pubmed
Khvorova,
Functional siRNAs and miRNAs exhibit strand bias.
2003,
Pubmed
Kim,
Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.
2005,
Pubmed
Lund,
Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
2011,
Pubmed
,
Xenbase
Lund,
Deadenylation of maternal mRNAs mediated by miR-427 in Xenopus laevis embryos.
2009,
Pubmed
,
Xenbase
Ma,
MicroRNA activity is suppressed in mouse oocytes.
2010,
Pubmed
MacRae,
In vitro reconstitution of the human RISC-loading complex.
2008,
Pubmed
Maniataki,
A human, ATP-independent, RISC assembly machine fueled by pre-miRNA.
2005,
Pubmed
Ni,
A genome-scale shRNA resource for transgenic RNAi in Drosophila.
2011,
Pubmed
O'Carroll,
A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway.
2007,
Pubmed
Paddison,
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.
2002,
Pubmed
Schwarz,
Asymmetry in the assembly of the RNAi enzyme complex.
2003,
Pubmed
Suh,
MicroRNA function is globally suppressed in mouse oocytes and early embryos.
2010,
Pubmed
Svoboda,
Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference.
2000,
Pubmed
Tam,
Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes.
2008,
Pubmed
Watanabe,
Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes.
2008,
Pubmed
Yang,
Dicer-independent, Ago2-mediated microRNA biogenesis in vertebrates.
2010,
Pubmed
Yang,
Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis.
2010,
Pubmed
Yi,
Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs.
2005,
Pubmed
Yigit,
Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi.
2006,
Pubmed
Zamore,
RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals.
2000,
Pubmed
Zhao,
Treatment with small interfering RNA affects the microRNA pathway and causes unspecific defects in zebrafish embryos.
2008,
Pubmed
