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CSH Protoc 2007 May 01;2007:pdb.prot4734. doi: 10.1101/pdb.prot4734.
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Inducing Ovulation in Xenopus laevis.

INTRODUCTIONThis protocol describes a method for inducing ovulation in Xenopus laevis for the purpose of in vitro fertilization. Ovulation is induced by injection of human chorionic gonadotropin (hCG) into the dorsal lymph sac of a female frog. Females can lay many hundreds of eggs. However, because the actual number of eggs laid and the efficiency of fertilization are unpredictable, ovulation should be induced in more than one female, even when only a few eggs are required.

PubMed ID: 21357079
Article link: CSH Protoc