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XB-ART-45880
Dev Biol 2012 Dec 01;3721:103-10. doi: 10.1016/j.ydbio.2012.08.013.
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Eif4a3 is required for accurate splicing of the Xenopus laevis ryanodine receptor pre-mRNA.

Haremaki T , Weinstein DC .


Abstract
The Exon Junction Complex (EJC) plays a critical role in multiple posttranscriptional events, including RNA subcellular localization, nonsense-mediated decay (NMD), and translation. We previously reported that knockdown of the EJC core component Eukaryotic initiation factor 4a3 (Eif4a3) results in full-body paralysis of embryos of the frog, Xenopus laevis. Here, we explore the cellular and molecular mechanisms underlying this phenotype. We find that cultured muscle cells derived from Eif4a3 morphants do not contract, and fail to undergo calcium-dependent calcium release in response to electrical stimulation or treatment with caffeine. We show that ryr (ryanodine receptor) transcripts are incorrectly spliced in Eif4a3 morphants, and demonstrate that inhibition of Xenopus Ryr function similarly results in embryonic paralysis. These results suggest that the EJC mediates muscle cell function via regulation of pre-mRNA splicing during early vertebrate embryogenesis.

PubMed ID: 22944195
PMC ID: PMC3479368
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: eif4a3.1 myc odc1 ryr1
Morpholinos: eif4a3.1 MO1 eif4a3.1 MO2 ryr1 MO1


Article Images: [+] show captions
References [+] :
Ashton-Beaucage, The exon junction complex controls the splicing of MAPK and other long intron-containing transcripts in Drosophila. 2010, Pubmed