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XB-ART-46161
Epigenetics Chromatin 2012 Oct 29;51:17. doi: 10.1186/1756-8935-5-17.
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HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes.

Jullien J , Astrand C , Szenker E , Garrett N , Almouzni G , Gurdon JB .


Abstract
UNLABELLED: BACKGROUND: Nuclear reprogramming is potentially important as a route to cell replacement and drug discovery, but little is known about its mechanism. Nuclear transfer to eggs and oocytes attempts to identify the mechanism of this direct route towards reprogramming by natural components. Here we analyze how the reprogramming of nuclei transplanted to Xenopus oocytes exploits the incorporation of the histone variant H3.3. RESULTS: After nuclear transplantation, oocyte-derived H3.3 but not H3.2, is deposited on several regions of the genome including rDNA, major satellite repeats, and the regulatory regions of Oct4. This major H3.3 deposition occurs in absence of DNA replication, and is HIRA-and transcription-dependent. It is necessary for the shift from a somatic- to an oocyte-type of transcription after nuclear transfer. CONCLUSIONS: This study demonstrates that the incorporation of histone H3.3 is an early and necessary step in the direct reprogramming of somatic cell nuclei by oocyte. It suggests that the incorporation of histone H3.3 is necessary during global changes in transcription that accompany changes in cell fate.

PubMed ID: 23102146
PMC ID: PMC3538669
Article link: Epigenetics Chromatin
Grant support: [+]

Species referenced: Xenopus
Genes referenced: cbx5 h3-3a hira mapt mtor pou5f3
Antibodies: h3-3b Ab1


Article Images: [+] show captions
References [+] :
Ahmad, The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly. 2002, Pubmed