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J Cell Biol 1988 Dec 01;1076 Pt 2:2657-67.
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Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.

Ingold AL , Cohn SA , Scholey JM .

We have prepared and characterized seven mouse monoclonal antibodies (SUK 1-7) to the 130-kD heavy chain of sea urchin egg kinesin. On immunoblots, SUK 3 and SUK 4 cross-reacted with Drosophila embryo 116-kD heavy chains, and SUK 4, SUK 5, SUK 6, and SUK 7 bound to the 120-kD heavy chains of bovine brain kinesin. Three out of seven monoclonal antikinesins (SUK 4, SUK 6, and SUK 7) caused a dose-dependent inhibition of sea urchin egg kinesin-induced microtubule translocation, whereas the other four monoclonal antibodies had no detectable effect on this motility. The inhibitory monoclonal antibodies (SUK 4, SUK 6, and SUK 7) appear to bind to spatially related sites on an ATP-sensitive microtubule binding 45-kD chymotryptic fragment of the 130-kD heavy chain, whereas SUK 2 binds to a spatially distinct site. None of the monoclonal antikinesins inhibited the microtubule activated MgATPase activity of kinesin, suggesting that SUK 4, SUK 6, and SUK 7 uncouple this MgATPase activity from motility.

PubMed ID: 2974459
PMC ID: PMC2115674

Species referenced: Xenopus
Genes referenced: kif1a kif5b
Antibodies: Kif1a Ab1 Kif5b Ab1 SUK 5 Ab1

References [+] :
Adams, Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I. , Pubmed