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Cell 2013 Jun 06;1536:1296-311. doi: 10.1016/j.cell.2013.05.004.
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Scaling of dorsal-ventral patterning by embryo size-dependent degradation of Spemann's organizer signals.

Inomata H , Shibata T , Haraguchi T , Sasai Y .

Spemann's organizer plays a key role in dorsal-ventral (DV) patterning in the amphibian embryo by secreting diffusible proteins such as Chordin, an antagonist to ventralizing bone morphogenetic proteins (BMPs). The DV patterning is so robust that an amphibian embryo with its ventral half surgically removed can develop into a smaller but proportionally patterned larva. Here, we show that this robust patterning depends on facilitated Chordin degradation and requires the expression of the Chordin-proteinase inhibitor Sizzled on the opposite side. Sizzled, which is stable and diffuses widely along the DV axis, stabilizes Chordin and expands its distribution in the ventral direction. This expanded Chordin distribution, in turn, limits BMP-dependent Sizzled production, forming an axis-wide feedback loop for shaping Chordin's activity. Using bisection assays, we demonstrate that Chordin degradation is dynamically controlled by embryo-size-coupled Sizzled accumulation. We propose a scaling model that enables the DV pattern to adjust proportionally to embryonic axis size.

PubMed ID: 23746842
Article link: Cell

Species referenced: Xenopus laevis
Genes referenced: admp babam2 bmp2 bmp4 bmp7.1 chrd egr2 mtor myod1 nog otx2 shh smad1 szl
Antibodies: Chrd Ab1 FLAG Ab1 GFP Ab10 Nog Ab1 Smad1 Ab7 Szl Ab1

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References :
De Robertis, Development: scaling to size by protease inhibition. 2013, Pubmed, Xenbase