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Int J Dev Biol 2013 Jan 01;575:407-14. doi: 10.1387/ijdb.130010st.
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Hippo signaling components, Mst1 and Mst2, act as a switch between self-renewal and differentiation in Xenopus hematopoietic and endothelial progenitors.

Hippo signaling is a conserved pathway that regulates cell proliferation and organ size control. Mst1 and Mst2 were identified as homologs of hippo and as core kinases of the Hippo pathway in mammals. Here, we have characterized the role of Mst1 and Mst2 during Xenopus primitive hematopoiesis. We showed that Mst1 and Mst2 were strongly expressed in the Xenopus ventral blood island, where primitive hematopoiesis is initiated. Loss-of-function analysis of Mst1/2 revealed morphogenetic defects, including short axis, smaller eyes and abnormal epidermis, and decreased phosphorylation of Yap. Mst1/2 morphants did not exhibit any change in the expression of hematopoietic and endothelial progenitor markers in early hematopoietic development. In addition, we have shown that such progenitor markers were continuously expressed through to the late hematopoietic development stage. As a result, the expression of erythroid, myeloid and endothelial differentiation markers were decreased in Mst1/2 morphants. Our results indicate that Mst1/2 act as a differentiation switch in Xenopus hematopoietc and endothelial progenitors.

PubMed ID: 23873372
Article link: Int J Dev Biol

Species referenced: Xenopus
Genes referenced: actl6a aplnr cebpa cfd etv2 fli1 gata1 gata2 hba1 hbg1 lmo2 mpo mst1 odc1 runx1 spib stk3 stk4 tal1 tek yap1
Antibodies: Cytoskeleton Ab1 HA Ab2 O-dianisidine Lectin 1 Yap1 Ab2 Yap1 Ab3
Morpholinos: stk3 MO1 stk3 MO2 stk4 MO1

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