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Tissue distribution and subcellular localization of mammalian myosin I.
Wagner MC
,
Barylko B
,
Albanesi JP
.
Abstract
Myosin I, a nonfilamentous single-headed actin-activated ATPase, has recently been purified from mammalian tissue (Barylko, B., M. C. Wagner, O. Reizes, and J. P. Albanesi. 1992. Proc. Natl. Acad. Sci. USA. 89:490-494). To investigate the distribution of this enzyme in cells and tissues mAbs were generated against myosin I purified from bovine adrenal gland. Eight antibodies were characterized, five of them (M4-M8) recognize epitope(s) on the catalytic "head" portion of myosin I while the other three (M1-M3) react with the "tail" domain. Immunoblot analysis using antiadrenal myosin I antibody M2 demonstrates the widespread distribution of the enzyme in mammalian tissues. Myosin I was immunolocalized in several cell types including bovine kidney (MDBK), rat kidney (NRK), rat brain, rat phaeochromocytoma (PC12), fibroblast (Swiss 3T3), and CHO cells. In all cases, myosin I was concentrated at the cell periphery. The most intense labeling was observed in regions of the cell usually associated with motile activity (i.e., filopodia, lamellipodia and growth cones). These results are consistent with earlier observations on protozoan myosin I that suggest a motile role for the enzyme at the plasma membrane.
Adams,
Binding of myosin I to membrane lipids.
1989, Pubmed
Adams,
Binding of myosin I to membrane lipids.
1989,
Pubmed
Baines,
Localization of myosin IC and myosin II in Acanthamoeba castellanii by indirect immunofluorescence and immunogold electron microscopy.
1990,
Pubmed
Barylko,
Purification and characterization of a mammalian myosin I.
1992,
Pubmed
Bikle,
Limited tissue distribution of the intestinal brush border myosin I protein.
1991,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brodsky,
Clathrin structure characterized with monoclonal antibodies. I. Analysis of multiple antigenic sites.
1986,
Pubmed
Coluccio,
Identification of the microvillar 110-kDa calmodulin complex (myosin-1) in kidney.
1992,
Pubmed
Coudrier,
Immunolocalization of the 110,000 molecular weight cytoskeletal protein of intestinal microvilli.
1982,
Pubmed
Fukui,
Myosin I is located at the leading edges of locomoting Dictyostelium amoebae.
1989,
Pubmed
Garcia,
Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.
1990,
Pubmed
Gillespie,
Chemiluminescence detection of proteins from single cells.
1991,
Pubmed
Glenney,
The intracellular localization of the microvillus 110K protein, a component considered to be involved in side-on membrane attachment of F-actin.
1982,
Pubmed
Hayden,
Binding of brush border myosin I to phospholipid vesicles.
1990,
Pubmed
Hoshimaru,
Immunochemical evidence that myosin I heavy chain-like protein is identical to the 110-kilodalton brush-border protein.
1990,
Pubmed
Hoshimaru,
Identification of a new type of mammalian myosin heavy chain by molecular cloning. Overlap of its mRNA with preprotachykinin B mRNA.
1987,
Pubmed
Höner,
Stress fiber dynamics as probed by antibodies against myosin.
1989,
Pubmed
Kawakami,
Structural organization and expression of the gene for bovine myosin I heavy chain.
1992,
Pubmed
Korn,
Myosin I.
1990,
Pubmed
Matsudaira,
Identification and organization of the components in the isolated microvillus cytoskeleton.
1980,
Pubmed
Matsudaira,
SDS microslab linear gradient polyacrylamide gel electrophoresis.
1978,
Pubmed
Meloche,
Molecular characterization of the solubilized atrial natriuretic factor receptor from bovine adrenal zona glomerulosa.
1987,
Pubmed
Mitchison,
Cytoskeletal dynamics and nerve growth.
1990,
Pubmed
Miyata,
Plasma membrane association of Acanthamoeba myosin I.
1989,
Pubmed
Pfister,
Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
1989,
Pubmed
Pollard,
Myosin-I.
1991,
Pubmed
Sheetz,
Concentration of membrane antigens by forward transport and trapping in neuronal growth cones.
1990,
Pubmed
Smith,
Neuronal cytomechanics: the actin-based motility of growth cones.
1988,
Pubmed
Tomasek,
Collagen modulates cell shape and cytoskeleton of embryonic corneal and fibroma fibroblasts: distribution of actin, alpha-actinin, and myosin.
1982,
Pubmed
